We report that in vivo increased acetylation of the repressed Saccharomyces cerevisiae ADH2 promoter chromatin, as obtained by disrupting the genes for the two deacetylases HDA1 and RPD3, destabilizes the structure of the TATA box-containing nucleosome. This acetylation-dependent chromatin remodeling is not sufficient to allow the binding of the TATA box-binding protein, but facilitates the recruitment of the transcriptional activator Adr1 and induces faster kinetics of mRNA accumulation when the cells are shifted to derepressing conditions.
Hyperacetylation of chromatin at the ADH2 promoter allows Adr1 to bind in repressed conditions / Verdone, Loredana; J. S., Wu; K., Van Riper; N., Kacherovsky; M., Vogelauer; E. T., Young; M., Grunstein; DI MAURO, Ernesto; M., Caserta. - In: EMBO JOURNAL. - ISSN 0261-4189. - STAMPA. - 21:5(2002), pp. 1101-1111. [10.1093/emboj/21.5.1101]
Hyperacetylation of chromatin at the ADH2 promoter allows Adr1 to bind in repressed conditions
VERDONE, Loredana;DI MAURO, Ernesto;
2002
Abstract
We report that in vivo increased acetylation of the repressed Saccharomyces cerevisiae ADH2 promoter chromatin, as obtained by disrupting the genes for the two deacetylases HDA1 and RPD3, destabilizes the structure of the TATA box-containing nucleosome. This acetylation-dependent chromatin remodeling is not sufficient to allow the binding of the TATA box-binding protein, but facilitates the recruitment of the transcriptional activator Adr1 and induces faster kinetics of mRNA accumulation when the cells are shifted to derepressing conditions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
