The aim of this study was to evaluate patterns of antiretroviral resistance of HIV-1 in peripheral blood mononuclear cells (PBMCs) and in the plasma of patients whose therapeutic regimen is failing. Plasma and PBMC samples were collected from 95 HIV-infected patients undergoing long-term treatment. Genotyping of the reverse transcriptase (RT) and protease genes of HIV-1 was undertaken using the fluorescent dideoxy-terminator method. Comparison of the amino acid sequence of the RT and protease genes in cell-associated variants of HIV-1 with that of the plasma revealed that 62 of the 95 patients' samples tested exhibited different genotypic resistance patterns (discordant samples [DSs]). In 27% of samples, the patterns of resistance detected were concordant in both compartments. In 51% of DSs, the greatest number of mutations was found in plasma; however, in 37% of DSs, greater numbers of mutations were found in PBMC DNA. The HIV mutation patterns detected in plasma do not necessarily reflect those found in the cell-associated compartment. The observation that the cellular compartment may contain an archive of the resistance variant makes this reservoir an interesting substrate for analysis of the "resistance potential" in a given patient.

Genotypic resistance of archived and circulating viral strains in the blood of treated HIV-infected individuals / Turriziani, Ombretta; Bucci, Mauro; Armando, Stano; Scagnolari, Carolina; Francesca, Bellomi; Caterina, Fimiani; Mezzaroma, Ivano; D'Ettorre, G.; Andrea, Brogi; Vullo, Vincenzo; Antonelli, Guido. - In: JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES. - ISSN 1525-4135. - STAMPA. - 44:5(2007), pp. 518-524. [10.1097/qai.0b013e3180315515]

Genotypic resistance of archived and circulating viral strains in the blood of treated HIV-infected individuals

TURRIZIANI, Ombretta;BUCCI, Mauro;SCAGNOLARI, CAROLINA;MEZZAROMA, Ivano;G. D'Ettorre;VULLO, Vincenzo;ANTONELLI, Guido
2007

Abstract

The aim of this study was to evaluate patterns of antiretroviral resistance of HIV-1 in peripheral blood mononuclear cells (PBMCs) and in the plasma of patients whose therapeutic regimen is failing. Plasma and PBMC samples were collected from 95 HIV-infected patients undergoing long-term treatment. Genotyping of the reverse transcriptase (RT) and protease genes of HIV-1 was undertaken using the fluorescent dideoxy-terminator method. Comparison of the amino acid sequence of the RT and protease genes in cell-associated variants of HIV-1 with that of the plasma revealed that 62 of the 95 patients' samples tested exhibited different genotypic resistance patterns (discordant samples [DSs]). In 27% of samples, the patterns of resistance detected were concordant in both compartments. In 51% of DSs, the greatest number of mutations was found in plasma; however, in 37% of DSs, greater numbers of mutations were found in PBMC DNA. The HIV mutation patterns detected in plasma do not necessarily reflect those found in the cell-associated compartment. The observation that the cellular compartment may contain an archive of the resistance variant makes this reservoir an interesting substrate for analysis of the "resistance potential" in a given patient.
drug resistance; hiv reservoirs; peripheral blood mononuclear cell; plasma
01 Pubblicazione su rivista::01a Articolo in rivista
Genotypic resistance of archived and circulating viral strains in the blood of treated HIV-infected individuals / Turriziani, Ombretta; Bucci, Mauro; Armando, Stano; Scagnolari, Carolina; Francesca, Bellomi; Caterina, Fimiani; Mezzaroma, Ivano; D'Ettorre, G.; Andrea, Brogi; Vullo, Vincenzo; Antonelli, Guido. - In: JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES. - ISSN 1525-4135. - STAMPA. - 44:5(2007), pp. 518-524. [10.1097/qai.0b013e3180315515]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/361963
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