Adult skeletal muscle contains a specialized population of myogenic quiescent stem cells, termed satellite cells, which contribute to repair myofibers after injury. During muscle regeneration, satellite cells exit their normal quiescent state, proliferate, activating MyoD and Myf-5 expression, and finally differentiate and fuse to reconstitute the injured muscle architecture. We have previously reported that cdk9 is required for myogenesis in vitro by activating MyoD-dependent transcription. In myoblasts induced to differentiate, MyoD recruits cdk9 on the chromatin of muscle-specific regulatory regions. This event correlates with chromatin-modifying enzyme recruitment and phosphorylation of cdk9-specific target residues at the carboxyl-terminal domain of RNA polymerase II. Here we report that a second cdk9 isoform, termed cdk9-55, plays a fundamental role in muscle regeneration and differentiation in vivo. This alternative form is specifically induced in injured myofibers and its activity is strictly required for the completion of muscle regeneration process.

Cdk9-55: a new player in muscle regeneration / Giacinti, Cristina; Musaro', Antonio; DE FALCO, G; Jourdan, I; Molinaro, Mario; Bagella, L; Simone, C; Giordano, A.. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - 216:(2008), pp. 576-582. [10.1002/jcp.21361]

Cdk9-55: a new player in muscle regeneration.

GIACINTI, Cristina;MUSARO', Antonio;MOLINARO, Mario;
2008

Abstract

Adult skeletal muscle contains a specialized population of myogenic quiescent stem cells, termed satellite cells, which contribute to repair myofibers after injury. During muscle regeneration, satellite cells exit their normal quiescent state, proliferate, activating MyoD and Myf-5 expression, and finally differentiate and fuse to reconstitute the injured muscle architecture. We have previously reported that cdk9 is required for myogenesis in vitro by activating MyoD-dependent transcription. In myoblasts induced to differentiate, MyoD recruits cdk9 on the chromatin of muscle-specific regulatory regions. This event correlates with chromatin-modifying enzyme recruitment and phosphorylation of cdk9-specific target residues at the carboxyl-terminal domain of RNA polymerase II. Here we report that a second cdk9 isoform, termed cdk9-55, plays a fundamental role in muscle regeneration and differentiation in vivo. This alternative form is specifically induced in injured myofibers and its activity is strictly required for the completion of muscle regeneration process.
2008
01 Pubblicazione su rivista::01a Articolo in rivista
Cdk9-55: a new player in muscle regeneration / Giacinti, Cristina; Musaro', Antonio; DE FALCO, G; Jourdan, I; Molinaro, Mario; Bagella, L; Simone, C; Giordano, A.. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - 216:(2008), pp. 576-582. [10.1002/jcp.21361]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/360996
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 17
social impact