The structure of a gene coding for bombinin-like peptides (BLP) in Bombina orientalis was determined. It comprises two exons separated by a 1337 bp intron. Exon 1 codes for the signal peptide, while exon 2 contains the genetic information for BLP-7 and a bombinin H-type peptide (GH-2). The promoter region contains putative recognition sites for nuclear factors, such as NF-IL6 and NF-kappaB. The analysis of the structure of this gene, compared with that of the previously reported BLP-3 gene sequence, suggests the occurrence of a gene duplication event, rather than an alternative splicing mechanism, which leads to the generation of both inter- and intra-families variability in this class of cytolytic peptides. Furthermore, walking chromosome analysis indicates that this gene family is not densely clustered. (C) 2000 Elsevier Science Inc. All rights reserved.

Sequence of a gene from Bombina orientalis coding for the antimicrobial peptide BLP-7 / Miele, Rossella; Borro, Marina; Fiocco, Daniela; Barra, Donatella; Simmaco, Maurizio. - In: PEPTIDES. - ISSN 0196-9781. - 21:11(2000), pp. 1681-1686. [10.1016/s0196-9781(00)00317-x]

Sequence of a gene from Bombina orientalis coding for the antimicrobial peptide BLP-7

MIELE, Rossella;BORRO, Marina;FIOCCO, Daniela;BARRA, Donatella;SIMMACO, Maurizio
2000

Abstract

The structure of a gene coding for bombinin-like peptides (BLP) in Bombina orientalis was determined. It comprises two exons separated by a 1337 bp intron. Exon 1 codes for the signal peptide, while exon 2 contains the genetic information for BLP-7 and a bombinin H-type peptide (GH-2). The promoter region contains putative recognition sites for nuclear factors, such as NF-IL6 and NF-kappaB. The analysis of the structure of this gene, compared with that of the previously reported BLP-3 gene sequence, suggests the occurrence of a gene duplication event, rather than an alternative splicing mechanism, which leads to the generation of both inter- and intra-families variability in this class of cytolytic peptides. Furthermore, walking chromosome analysis indicates that this gene family is not densely clustered. (C) 2000 Elsevier Science Inc. All rights reserved.
2000
amphibia; antimicrobial peptide gene; bombina orientalis; bombinin (blp); bombinin h
01 Pubblicazione su rivista::01a Articolo in rivista
Sequence of a gene from Bombina orientalis coding for the antimicrobial peptide BLP-7 / Miele, Rossella; Borro, Marina; Fiocco, Daniela; Barra, Donatella; Simmaco, Maurizio. - In: PEPTIDES. - ISSN 0196-9781. - 21:11(2000), pp. 1681-1686. [10.1016/s0196-9781(00)00317-x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/360889
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