CZE separation of nitrogenous drugs in cationic form

A rapid and efficient capillary zone electrophoresis method was developed for Quality Control analysis of pharmaceutical preparations containing antihistamines, decongestants and anticholinergic remedies. chlorpheniramine, diphenhydramine, ephedrine. isopropamide, pheniramine. These analytes were separated in cationic form optimizing the experimental conditions in 60 mM tetraborate buffer pH = 9 2 as a BGE (Background Electrolyte) on a Beckman P/ACE System MDQ instrument. The effective capillary length was 48 cm, I D. = 75 mu m, the applied voltage 15 kV, and the temperature 25 degrees C. Detection was performed by a DAD (Diode Array Detector) at 210 nm Separation time was less than 8 min After experimental conditions optimization, the proposed method was validated Precision of migration time (t(m)) ranging from 0 19% to 0 29% and corrected peak area (A(c)) from 2.54% to 3 680,4 The linearity of detector response was tested in the range 5-40 mu g ml(-1) obtaining the 0.9962 <= r(2) 9982 LOD and LOQ, accuracy (recovery) and ruggedness were evaluated for each analyte demonstrating the good reliability of the method Analysis of the pharmaceutical real sample was performed