Human papillomaviruses (HPVs) and herpes simplex virus type 2 (HSV-2) can establish latent or persistent infections in the host, and are involved in the aetiology of benign and/or malignant lesions of the urogenital tract. To investigate the putative interaction between these DNA viruses when a double infection occurs, we have studied the effect of HSV-2 infection in HeLa 229 cells containing 10-50 copies of HPV type 18 genomic DNA. Twenty hours post HSV-2 infection, the analysis of mRNA transcripts from E1, E2, E6 early and L1 late HPV18 genes was performed in HeLa cells by a semi-quantitative RT-PCR assay. A modulation of HPV18 E1 and E6 early genes was observed, resulting in a 9-fold and 3-fold increased transcription respectively.

Involvement of herpes simplex virus type 2 in modulation of gene expression of human papillomavirus type 18 / Pisani, S; Fioriti, Daniela; Conte, Maria Pia; Chiarini, Fernanda; Seganti, Lucilla; Degener, Anna Marta. - In: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY. - ISSN 0394-6320. - STAMPA. - 15.1:(2002), pp. 59-63.

Involvement of herpes simplex virus type 2 in modulation of gene expression of human papillomavirus type 18

FIORITI, DANIELA;CONTE, Maria Pia;CHIARINI, Fernanda;SEGANTI, Lucilla;DEGENER, Anna Marta
2002

Abstract

Human papillomaviruses (HPVs) and herpes simplex virus type 2 (HSV-2) can establish latent or persistent infections in the host, and are involved in the aetiology of benign and/or malignant lesions of the urogenital tract. To investigate the putative interaction between these DNA viruses when a double infection occurs, we have studied the effect of HSV-2 infection in HeLa 229 cells containing 10-50 copies of HPV type 18 genomic DNA. Twenty hours post HSV-2 infection, the analysis of mRNA transcripts from E1, E2, E6 early and L1 late HPV18 genes was performed in HeLa cells by a semi-quantitative RT-PCR assay. A modulation of HPV18 E1 and E6 early genes was observed, resulting in a 9-fold and 3-fold increased transcription respectively.
2002
01 Pubblicazione su rivista::01a Articolo in rivista
Involvement of herpes simplex virus type 2 in modulation of gene expression of human papillomavirus type 18 / Pisani, S; Fioriti, Daniela; Conte, Maria Pia; Chiarini, Fernanda; Seganti, Lucilla; Degener, Anna Marta. - In: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY. - ISSN 0394-6320. - STAMPA. - 15.1:(2002), pp. 59-63.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/359882
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