Background: Celiac disease (CD) is a complex disorder triggered by gluten affecting genetically predisposed individuals. More than 90% of patients carry human lymphocyte antigen (HLA)-DQ2 (DQA1*05, DOB1*02) and/or HLA-DQ8 (DQA1*03, DOB1*0302). We propose the use of the DQ-CD Typing kit that allows identification of the HLA class II alleles (DQA1*0201,*03,*05, DQB1*02,*0302, DRB1*03,*04, *07) selected to be informative in the CID risk evaluation and of a second kit, namely DQ-CD Zygosis, for DOB1*02 homozygosity determination. Methods: The study was performed on a cohort of 100 individuals previously HLA typed with commercial kits. Fresh blood or previously extracted DNA was amplified in a unique PCR program using allele-specific primers and visualized on agarose gel. Results: DNA amplification yielded strong and clear products without non specific signals or ghost bands. All the samples showed the expected alleles in accordance with the previous HLA typing. Conclusions: The DQ-CD Typing and Zygosis kits are fast, simple, economical and accurate tools that can be used to determinate the HLA-DQ2/DQ8 status in laboratory practice addressed for the diagnosis of CID. Molecular HLA testing is considered a valid support in the confirmation/exclusion of CID, especially in high-risk groups, such as CID relatives, or when serological and histological data are ambiguous.

A rapid and sensitive method to detect specific human lymphocyte antigen (HLA) class II alleles associated with celiac disease / Megiorni, Francesca; Barbara, Mora; Bonamico, Margherita; Nenna, Raffaella; Mariarosaria Di, Pierro; Carlo, Catassi; Sandro, Drago; Mazzilli, Maria Cristina. - STAMPA. - 46:2(2008), pp. 193-196. [10.1515/cclm.2008.049]

A rapid and sensitive method to detect specific human lymphocyte antigen (HLA) class II alleles associated with celiac disease

MEGIORNI, Francesca;BONAMICO, Margherita;NENNA, RAFFAELLA;MAZZILLI, Maria Cristina
2008

Abstract

Background: Celiac disease (CD) is a complex disorder triggered by gluten affecting genetically predisposed individuals. More than 90% of patients carry human lymphocyte antigen (HLA)-DQ2 (DQA1*05, DOB1*02) and/or HLA-DQ8 (DQA1*03, DOB1*0302). We propose the use of the DQ-CD Typing kit that allows identification of the HLA class II alleles (DQA1*0201,*03,*05, DQB1*02,*0302, DRB1*03,*04, *07) selected to be informative in the CID risk evaluation and of a second kit, namely DQ-CD Zygosis, for DOB1*02 homozygosity determination. Methods: The study was performed on a cohort of 100 individuals previously HLA typed with commercial kits. Fresh blood or previously extracted DNA was amplified in a unique PCR program using allele-specific primers and visualized on agarose gel. Results: DNA amplification yielded strong and clear products without non specific signals or ghost bands. All the samples showed the expected alleles in accordance with the previous HLA typing. Conclusions: The DQ-CD Typing and Zygosis kits are fast, simple, economical and accurate tools that can be used to determinate the HLA-DQ2/DQ8 status in laboratory practice addressed for the diagnosis of CID. Molecular HLA testing is considered a valid support in the confirmation/exclusion of CID, especially in high-risk groups, such as CID relatives, or when serological and histological data are ambiguous.
2008
celiac disease; dq2/dq8 molecules; human lymphocyte antigen (hla) typing; sequence specific primer-polymerase chain reaction (ssp-pcr)
01 Pubblicazione su rivista::01a Articolo in rivista
A rapid and sensitive method to detect specific human lymphocyte antigen (HLA) class II alleles associated with celiac disease / Megiorni, Francesca; Barbara, Mora; Bonamico, Margherita; Nenna, Raffaella; Mariarosaria Di, Pierro; Carlo, Catassi; Sandro, Drago; Mazzilli, Maria Cristina. - STAMPA. - 46:2(2008), pp. 193-196. [10.1515/cclm.2008.049]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/359272
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