XendoU is the endoribonuclease involved in the biosynthesis of a specific subclass of Xenopus laevis intron-encoded small nucleolar RNAs. XendoU has no homology to any known cellular RNase, although it has sequence similarity with proteins tentatively annotated as serine proteases. It has been recently shown that XendoU represents the cellular counterpart of a nidovirus replicative endoribonuclease (NendoU), which plays a critical role in viral replication and transcription. In this paper, we combined prediction and experimental data to define the amino acid residues directly involved in XendoU catalysis. Specifically, we find that XendoU residues Glu-161, Glu-167, His-162, His-178, and Lys-224 are essential for RNA cleavage, which occurs in the presence of manganese ions. Furthermore, we identified the RNA sequence required for XendoU binding and showed that the formation of XendoU-RNA complex is Mn2+-independent.

Functional characterisation of XendoU, the endoribonuclease involved in small nucleolar RNA biosynthesis / Gioia, Ubaldo; P., Laneve; Dlakic, M; Arceci, M; Bozzoni, Irene; Caffarelli, E.. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - STAMPA. - 280:(2005), pp. 18996-19002. [10.1074/jbc.M501160200]

Functional characterisation of XendoU, the endoribonuclease involved in small nucleolar RNA biosynthesis

GIOIA, UBALDO;BOZZONI, Irene;
2005

Abstract

XendoU is the endoribonuclease involved in the biosynthesis of a specific subclass of Xenopus laevis intron-encoded small nucleolar RNAs. XendoU has no homology to any known cellular RNase, although it has sequence similarity with proteins tentatively annotated as serine proteases. It has been recently shown that XendoU represents the cellular counterpart of a nidovirus replicative endoribonuclease (NendoU), which plays a critical role in viral replication and transcription. In this paper, we combined prediction and experimental data to define the amino acid residues directly involved in XendoU catalysis. Specifically, we find that XendoU residues Glu-161, Glu-167, His-162, His-178, and Lys-224 are essential for RNA cleavage, which occurs in the presence of manganese ions. Furthermore, we identified the RNA sequence required for XendoU binding and showed that the formation of XendoU-RNA complex is Mn2+-independent.
2005
01 Pubblicazione su rivista::01a Articolo in rivista
Functional characterisation of XendoU, the endoribonuclease involved in small nucleolar RNA biosynthesis / Gioia, Ubaldo; P., Laneve; Dlakic, M; Arceci, M; Bozzoni, Irene; Caffarelli, E.. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - STAMPA. - 280:(2005), pp. 18996-19002. [10.1074/jbc.M501160200]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/359173
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