In Burkitt's lymphoma cells, Epstein Barr virus (EBV) latency products interact with the ubiquitin-proteasome system to promote episomal maintenance and immunological evasion while the tripeptidylpeptidase II (TPPII) functions as an alternative protease. In the present study, we have examined the activities and levels of the proteasome and TPPII complex in Raji and in Akata cells after induction of EBV lytic cycle. The results show that the chymotrypsin-like and caspase-like activities of the proteasome were substantially reduced in Raji and Akata cells. Similarly, TPPII activity was diminished in both cell lines but was recovered in Akata cells at longer time after induction. Protein levels of the alpha/beta subunits of the 20S proteasome and TPPII concentration decreased to different extents after EBV activation, whereas the ubiquitin binding S6' subunit of the 19S regulatory complex increased three to fourfold along with the levels of ubiquitin-conjugates. Collectively, these observations demonstrate impairment of two major cellular proteolytic systems at the onset of EBV lytic infection. (c) 2006 Elsevier Inc. All rights reserved.

Down-regulation of proteolytic complexes following EBV activation in BL cells / Giulia, Matusali; DE LEO, Alessandra; Riccardo, Gavioli; Laura, Bertelli; DI RENZO, Livia Maria; Mattia, Elena. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 352:4(2007), pp. 947-952. [10.1016/j.bbrc.2006.11.127]

Down-regulation of proteolytic complexes following EBV activation in BL cells

DE LEO, ALESSANDRA;DI RENZO, Livia Maria;MATTIA, Elena
2007

Abstract

In Burkitt's lymphoma cells, Epstein Barr virus (EBV) latency products interact with the ubiquitin-proteasome system to promote episomal maintenance and immunological evasion while the tripeptidylpeptidase II (TPPII) functions as an alternative protease. In the present study, we have examined the activities and levels of the proteasome and TPPII complex in Raji and in Akata cells after induction of EBV lytic cycle. The results show that the chymotrypsin-like and caspase-like activities of the proteasome were substantially reduced in Raji and Akata cells. Similarly, TPPII activity was diminished in both cell lines but was recovered in Akata cells at longer time after induction. Protein levels of the alpha/beta subunits of the 20S proteasome and TPPII concentration decreased to different extents after EBV activation, whereas the ubiquitin binding S6' subunit of the 19S regulatory complex increased three to fourfold along with the levels of ubiquitin-conjugates. Collectively, these observations demonstrate impairment of two major cellular proteolytic systems at the onset of EBV lytic infection. (c) 2006 Elsevier Inc. All rights reserved.
2007
epstein barr virus; lytic cycle; proteasome; tppii
01 Pubblicazione su rivista::01a Articolo in rivista
Down-regulation of proteolytic complexes following EBV activation in BL cells / Giulia, Matusali; DE LEO, Alessandra; Riccardo, Gavioli; Laura, Bertelli; DI RENZO, Livia Maria; Mattia, Elena. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 352:4(2007), pp. 947-952. [10.1016/j.bbrc.2006.11.127]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/359005
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