Drought is an abiotic stress that strongly influences plant growth, development and productivity. By proteomics study it is possible to identify the complex mechanism of water-stress response. The aim of this research was the analysis of wheat (Triticum durum) proteome changes under stress conditions by applying a severe water deficit treatment for 7 days. Stress-induced proteome changes were analyzed by two-dimensional gel electrophoresis in conjunction with matrix-assisted laser desorption ionizationtime of flight mass spectrometry. Thirty-six protein spots showed a reproducible significant change between control and stressed samples. The reasonable implications in drought response of the identified proteins were discussed. Results provide new insights that can lead to a better understanding of the molecular basis of drought-sensitivity in plants. Therefore, the obtained data could suggest the development of drought resistant wheat varieties, in order to improve agricultural production in dry regions.
Analysis of drought responsive proteins in wheat (Triticum durum) by 2D-PAGE and MALDI-TOF mass spectrometry / Caruso, Giuseppe; Cavaliere, Chiara; Foglia, Patrizia; Gubbiotti, Riccardo; Samperi, Roberto; Lagana', Aldo. - In: PLANT SCIENCE. - ISSN 0168-9452. - STAMPA. - 177:6(2009), pp. 570-576. [10.1016/j.plantsci.2009.08.007]
Analysis of drought responsive proteins in wheat (Triticum durum) by 2D-PAGE and MALDI-TOF mass spectrometry
CARUSO, Giuseppe;CAVALIERE, CHIARA;FOGLIA, Patrizia;GUBBIOTTI, RICCARDO;SAMPERI, Roberto;LAGANA', Aldo
2009
Abstract
Drought is an abiotic stress that strongly influences plant growth, development and productivity. By proteomics study it is possible to identify the complex mechanism of water-stress response. The aim of this research was the analysis of wheat (Triticum durum) proteome changes under stress conditions by applying a severe water deficit treatment for 7 days. Stress-induced proteome changes were analyzed by two-dimensional gel electrophoresis in conjunction with matrix-assisted laser desorption ionizationtime of flight mass spectrometry. Thirty-six protein spots showed a reproducible significant change between control and stressed samples. The reasonable implications in drought response of the identified proteins were discussed. Results provide new insights that can lead to a better understanding of the molecular basis of drought-sensitivity in plants. Therefore, the obtained data could suggest the development of drought resistant wheat varieties, in order to improve agricultural production in dry regions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
