In order to define a cellular model suitable for studying, in vitro, the molecular properties and functions of neurotrophin receptors in human lymphocytes, TrkA, TrkB, TrkC and p75(NTR) expression was investigated in a panel of EBV immortalized lymphoblastoid (LCL) and Burkitt lymphoma-derived cell lines (BLs) compared to primary B lymphocytes by RT-PCR and flow cytometric analysis, Our data show that trkA and trkB are transcribed in most B cell lines of normal and malignant origin, For several of them, we also gained first evidence of trkC expression in B cells. All cell lines and primary B cells lack p75(NTR) expression. These data suggest that neurotrophin receptors expression in the B cell lines correlates to some extent with the phenotypic maturation stage and endogenous viral activity levels, Our data suggest that TrkA and TrkB, once activated, provide a partial rescue from apoptosis, whereas TrkC stimulates the progression through the cell cycle without affecting cell survival. Finally, the identification of a number of cell lines showing single expression of one of the Trk receptors has disclosed the availability of a cellular tool for further studies on their function, and mechanisms of signal transduction in the B cell moiety in the absence of p75(NTR).

Expression of neurotrophin receptors in normal and malignant B lymphocytes / D'Onofrio, Mara; DE GRAZIA, Ugo; Morrone, Stefania; Cuomo, Laura; Spinsanti, Paola; Frati, Luigi; Gulino, Alberto; Ragona, Giuseppe. - In: EUROPEAN CYTOKINE NETWORK. - ISSN 1148-5493. - STAMPA. - 11:2(2000), pp. 283-291.

Expression of neurotrophin receptors in normal and malignant B lymphocytes

D'ONOFRIO, Mara;DE GRAZIA, Ugo;MORRONE, Stefania;CUOMO, Laura;SPINSANTI, Paola;FRATI, Luigi;GULINO, Alberto;RAGONA, Giuseppe
2000

Abstract

In order to define a cellular model suitable for studying, in vitro, the molecular properties and functions of neurotrophin receptors in human lymphocytes, TrkA, TrkB, TrkC and p75(NTR) expression was investigated in a panel of EBV immortalized lymphoblastoid (LCL) and Burkitt lymphoma-derived cell lines (BLs) compared to primary B lymphocytes by RT-PCR and flow cytometric analysis, Our data show that trkA and trkB are transcribed in most B cell lines of normal and malignant origin, For several of them, we also gained first evidence of trkC expression in B cells. All cell lines and primary B cells lack p75(NTR) expression. These data suggest that neurotrophin receptors expression in the B cell lines correlates to some extent with the phenotypic maturation stage and endogenous viral activity levels, Our data suggest that TrkA and TrkB, once activated, provide a partial rescue from apoptosis, whereas TrkC stimulates the progression through the cell cycle without affecting cell survival. Finally, the identification of a number of cell lines showing single expression of one of the Trk receptors has disclosed the availability of a cellular tool for further studies on their function, and mechanisms of signal transduction in the B cell moiety in the absence of p75(NTR).
2000
b lymphocytes; bls; ebv; lcl; rt-pcr; trk
01 Pubblicazione su rivista::01a Articolo in rivista
Expression of neurotrophin receptors in normal and malignant B lymphocytes / D'Onofrio, Mara; DE GRAZIA, Ugo; Morrone, Stefania; Cuomo, Laura; Spinsanti, Paola; Frati, Luigi; Gulino, Alberto; Ragona, Giuseppe. - In: EUROPEAN CYTOKINE NETWORK. - ISSN 1148-5493. - STAMPA. - 11:2(2000), pp. 283-291.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/358192
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