Inflammation plays a crucial role in the development and progression of atherosclerotic plaques. The aim of this study is to compare culture supernatants from uncomplicated and complicated carotid atherosclerotic plaques by a multiplex approach, to assess the molecular mediators associated with a complicated plaque phenotype. Atherosclerotic plaques were obtained from 17 patients undergoing carotid endarterectomy. Supernatants from plaque cultures were evaluated by Bio-Plex cytokine assay to determine 27 pro- and anti-inflammatory cytokines, chemokines and growth factors. Complicated plaques secreted higher levels of IP-10 (p = 0.027) and lower levels of IL-5 (p = 0.045) than did uncomplicated ones. Distinctive secretory patterns of cytokines, chemokines and growth factors were present in the two types of plaque. Our study identifies IP-10 and IL-5 as proteins differentiating complicated and uncomplicated plaques from human carotid arteries and provides new insights into the interplay of molecular mediators with atherosclerotic plaque progression.
IDENTIFICATION OF IP-10 AND IL-5 AS PROTEINS DIFFERENTIALLY EXPRESSED IN HUMAN COMPLICATED AND UNCOMPLICATED CAROTID ATHEROSCLEROTIC PLAQUES / E., Profumo; B., Buttari; M. E., Tosti; Alessandri, Cristiano; Valesini, Guido; L., Marcuccio; C., Tesori; Capoano, Raffaele; Salvati, Bruno; R., Rigano. - In: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY. - ISSN 0394-6320. - STAMPA. - 23:3(2010), pp. 775-782.
IDENTIFICATION OF IP-10 AND IL-5 AS PROTEINS DIFFERENTIALLY EXPRESSED IN HUMAN COMPLICATED AND UNCOMPLICATED CAROTID ATHEROSCLEROTIC PLAQUES
ALESSANDRI, cristiano;VALESINI, Guido;CAPOANO, Raffaele;SALVATI, Bruno;
2010
Abstract
Inflammation plays a crucial role in the development and progression of atherosclerotic plaques. The aim of this study is to compare culture supernatants from uncomplicated and complicated carotid atherosclerotic plaques by a multiplex approach, to assess the molecular mediators associated with a complicated plaque phenotype. Atherosclerotic plaques were obtained from 17 patients undergoing carotid endarterectomy. Supernatants from plaque cultures were evaluated by Bio-Plex cytokine assay to determine 27 pro- and anti-inflammatory cytokines, chemokines and growth factors. Complicated plaques secreted higher levels of IP-10 (p = 0.027) and lower levels of IL-5 (p = 0.045) than did uncomplicated ones. Distinctive secretory patterns of cytokines, chemokines and growth factors were present in the two types of plaque. Our study identifies IP-10 and IL-5 as proteins differentiating complicated and uncomplicated plaques from human carotid arteries and provides new insights into the interplay of molecular mediators with atherosclerotic plaque progression.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
