Background/Aims: Estrogen receptors (ER) in cholangiocytes of primary biliary cirrhosis (PBC) patients and their relationship with cell proliferation and death were evaluated. Methods: Liver biopsies from PBC patients with different histological stages were investigated by immunohistochemistry for ER-alpha and -beta, cytokeratin-19, proliferating cellular nuclear antigen (PCNA), Fas and terminal deoxynucleotide transferase end labelling (TUNEL). Normal livers and livers from primary sclerosing cholangitis and alcoholic cirrhosis were investigated as controls. Results: ER-alpha and -beta were observed in cholangiocytes of PBC patients but not in normal liver. In PBC, positivity for ER-beta was high (50-65 %) in all histological stages while, positivity for ER-alpha increased from 1 % in stage I to 12 % in stage III (positivity correlated and co-localized in the same cell with PCNA). In stage IV of PBC, cholangiocytes were negative for ER-alpha in association with a lower PCNA positivity and with maximal degree of ductopenia. ER-alpha positivity in cholangiocytes of PBC patients was markedly lower than primary sclerosing cholangitis and alcoholic cirrhosis. Conclusions: ER are expressed in PBC and other pathologies associated with cholangiocyte proliferation but not in normal subjects. The low expression of ER-alpha in PBC and their disappearance in the advanced histological stages suggests that an estrogenic deficiency could favour the evolution of this disease toward ductopenia. (C) 2004 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.

Estrogen receptors in cholangiocytes and the progression of primary biliary cirrhosis / Alvaro, Domenico; Pietro, Invernizzi; Onori, Paolo; Franchitto, Antonio; DE SANTIS, Adriano; A., Crossignani; Roberta, Sferra; GINANNI CORRADINI, Stefano; M. G., Mancino; Marco, Maggioni; Attili, Adolfo Francesco; Mauro, Podda; Gaudio, Eugenio. - In: JOURNAL OF HEPATOLOGY. - ISSN 0168-8278. - STAMPA. - 41:6(2004), pp. 905-912. [10.1016/j.jhep.2004.08.022]

Estrogen receptors in cholangiocytes and the progression of primary biliary cirrhosis

ALVARO, Domenico;ONORI, PAOLO;FRANCHITTO, Antonio;DE SANTIS, Adriano;GINANNI CORRADINI, Stefano;ATTILI, Adolfo Francesco;GAUDIO, EUGENIO
2004

Abstract

Background/Aims: Estrogen receptors (ER) in cholangiocytes of primary biliary cirrhosis (PBC) patients and their relationship with cell proliferation and death were evaluated. Methods: Liver biopsies from PBC patients with different histological stages were investigated by immunohistochemistry for ER-alpha and -beta, cytokeratin-19, proliferating cellular nuclear antigen (PCNA), Fas and terminal deoxynucleotide transferase end labelling (TUNEL). Normal livers and livers from primary sclerosing cholangitis and alcoholic cirrhosis were investigated as controls. Results: ER-alpha and -beta were observed in cholangiocytes of PBC patients but not in normal liver. In PBC, positivity for ER-beta was high (50-65 %) in all histological stages while, positivity for ER-alpha increased from 1 % in stage I to 12 % in stage III (positivity correlated and co-localized in the same cell with PCNA). In stage IV of PBC, cholangiocytes were negative for ER-alpha in association with a lower PCNA positivity and with maximal degree of ductopenia. ER-alpha positivity in cholangiocytes of PBC patients was markedly lower than primary sclerosing cholangitis and alcoholic cirrhosis. Conclusions: ER are expressed in PBC and other pathologies associated with cholangiocyte proliferation but not in normal subjects. The low expression of ER-alpha in PBC and their disappearance in the advanced histological stages suggests that an estrogenic deficiency could favour the evolution of this disease toward ductopenia. (C) 2004 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
2004
apoptosis; bile-ducts; breast-cancer; epithelial-cells; er-beta; expression; liver-disease; nonsuppurative destructive cholangitis; proliferation; women
01 Pubblicazione su rivista::01a Articolo in rivista
Estrogen receptors in cholangiocytes and the progression of primary biliary cirrhosis / Alvaro, Domenico; Pietro, Invernizzi; Onori, Paolo; Franchitto, Antonio; DE SANTIS, Adriano; A., Crossignani; Roberta, Sferra; GINANNI CORRADINI, Stefano; M. G., Mancino; Marco, Maggioni; Attili, Adolfo Francesco; Mauro, Podda; Gaudio, Eugenio. - In: JOURNAL OF HEPATOLOGY. - ISSN 0168-8278. - STAMPA. - 41:6(2004), pp. 905-912. [10.1016/j.jhep.2004.08.022]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/357963
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