Transcription factors (TFs) are regulatory proteins that lime played a pivotal role in the evolution of eukaryotes and that also have great biotechnological potential. REGIA (REgulatory Gene Initiative in Arabidopsis) is an EU-funded project involving 29 European laboratories with the objective of determining the function of virtually all transcription factors from the model plant, Arabidopsis thaliana. REGIA involves: 1. the definition of TF gene expression patterns in Arabidopsis, 2. the identification of mutations at TF loci; 3. the ectopic expression of TFs (or derivatives) in Arabidopsis and in crop plants; 4. phenotypic analysis of the mutants and mis-expression lines, including both RNA and metabolic profiling; 5. the systematic analysis of interactions between TFs; and 6. the generation of a bioinformatics infrastructure to access and integrate all this information. We expect that this programme will establish the full biotechnological potential of plant TFs, and provide insights into hierarchies, redundancies, and interdependencies, and their evolution. The project involves the preparation of both a TF gene array for expression analysis and a normalised full length open reading frame (ORF) library of TFs in a yeast two hybrid vector; the applications of these resources should extend beyond the scope of this programme. Copyright (C) 2002 John Wiley Sons, Ltd.

REGIA, an EU project on functional genomics of transcription factors from Arabidopsis thaliana / Javier Paz, Ares; A., Valencia; Costantino, Paolo; Vittorioso, Paola; B., Davies; P., Gilmartin; J., Giraudat; F., Parcy; A., Reindl; R., Sablowski; G., Coupland; C., Martin; G. C., Angenent; H., Baumlein; Hans P., Mock; P., Carbonero; L., Colombo; C., Tonelli; P., Engstrom; W., Droege Laser; C., Gatz; T., Kavanagh; S., Kushnir; M., Zabeau; T., Laux; M., Hordsworth; I., Ruberti; F., Ratcliff; S., Smeekens; I., Somssich; B., Weisshaar; J., Traas. - In: COMPARATIVE AND FUNCTIONAL GENOMICS. - ISSN 1531-6912. - ELETTRONICO. - 3:2(2002), pp. 102-108. [10.1002/cfg.146]

REGIA, an EU project on functional genomics of transcription factors from Arabidopsis thaliana

COSTANTINO, Paolo;VITTORIOSO, Paola;
2002

Abstract

Transcription factors (TFs) are regulatory proteins that lime played a pivotal role in the evolution of eukaryotes and that also have great biotechnological potential. REGIA (REgulatory Gene Initiative in Arabidopsis) is an EU-funded project involving 29 European laboratories with the objective of determining the function of virtually all transcription factors from the model plant, Arabidopsis thaliana. REGIA involves: 1. the definition of TF gene expression patterns in Arabidopsis, 2. the identification of mutations at TF loci; 3. the ectopic expression of TFs (or derivatives) in Arabidopsis and in crop plants; 4. phenotypic analysis of the mutants and mis-expression lines, including both RNA and metabolic profiling; 5. the systematic analysis of interactions between TFs; and 6. the generation of a bioinformatics infrastructure to access and integrate all this information. We expect that this programme will establish the full biotechnological potential of plant TFs, and provide insights into hierarchies, redundancies, and interdependencies, and their evolution. The project involves the preparation of both a TF gene array for expression analysis and a normalised full length open reading frame (ORF) library of TFs in a yeast two hybrid vector; the applications of these resources should extend beyond the scope of this programme. Copyright (C) 2002 John Wiley Sons, Ltd.
2002
arabidopsis; arabidopsis thaliana; array; dof family; functional genomics; insertion mutant; regia; transcription factors; two-hybrid system
01 Pubblicazione su rivista::01a Articolo in rivista
REGIA, an EU project on functional genomics of transcription factors from Arabidopsis thaliana / Javier Paz, Ares; A., Valencia; Costantino, Paolo; Vittorioso, Paola; B., Davies; P., Gilmartin; J., Giraudat; F., Parcy; A., Reindl; R., Sablowski; G., Coupland; C., Martin; G. C., Angenent; H., Baumlein; Hans P., Mock; P., Carbonero; L., Colombo; C., Tonelli; P., Engstrom; W., Droege Laser; C., Gatz; T., Kavanagh; S., Kushnir; M., Zabeau; T., Laux; M., Hordsworth; I., Ruberti; F., Ratcliff; S., Smeekens; I., Somssich; B., Weisshaar; J., Traas. - In: COMPARATIVE AND FUNCTIONAL GENOMICS. - ISSN 1531-6912. - ELETTRONICO. - 3:2(2002), pp. 102-108. [10.1002/cfg.146]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/357205
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