Gene expression profiling has the potential to enhance current methods for the diagnosis of haematological malignancies. Here, we present data on 204 analyses from an international standardization programme that was conducted in 11 laboratories as a prephase to the Microarray Innovations in LEukemia (MILE) study. Each laboratory prepared two cell line samples, together with three replicate leukaemia patient lysates in two distinct stages: (i) a 5-d course of protocol training, and (ii) independent proficiency testing. Unsupervised, supervised, and r(2) correlation analyses demonstrated that microarray analysis can be performed with remarkably high intra-laboratory reproducibility and with comparable quality and reliability.
An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase / Alexander, Kohlmann; Thomas J., Kipps; Laura Z., Rassenti; James R., Downing; Sheila A., Shurtleff; Ken I., Mills; Amanda F., Gilkes; Wolf Karsten, Hofmann; Giuseppe, Basso; Marta Campo, Dell'Orto; Foa, Roberto; Chiaretti, Sabina; John De, Vos; Sonja, Rauhut; Peter R., Papenhausen; Jesus M., Hernandez; Eva, Lumbreras; Allen E., Yeoh; Evelyn S., Koay; Rachel, Li; Wei Min, Liu; Paul M., Williams; Lothar, Wieczorek; Torsten, Haferlach. - In: BRITISH JOURNAL OF HAEMATOLOGY. - ISSN 0007-1048. - STAMPA. - 142:5(2008), pp. 802-807. [10.1111/j.1365-2141.2008.07261.x]
An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase
FOA, Roberto;CHIARETTI, sabina;
2008
Abstract
Gene expression profiling has the potential to enhance current methods for the diagnosis of haematological malignancies. Here, we present data on 204 analyses from an international standardization programme that was conducted in 11 laboratories as a prephase to the Microarray Innovations in LEukemia (MILE) study. Each laboratory prepared two cell line samples, together with three replicate leukaemia patient lysates in two distinct stages: (i) a 5-d course of protocol training, and (ii) independent proficiency testing. Unsupervised, supervised, and r(2) correlation analyses demonstrated that microarray analysis can be performed with remarkably high intra-laboratory reproducibility and with comparable quality and reliability.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
