The synthesis of a new chiral agent, (R,R) (-)N, N'-trans-1, 2-dihexylcyclohexanediamine, for the chromatographic resolution of racemates is reported. Highly selective separations of D- and L-isomers of free and Dns-amino acids were accomplished on a reversed-phase column using in the mobile phase a Cu(II) complex of the above chiral selector. The procedure was extended to resolve diastereomeric derivatives, which were obtained by reaction of an optically active amine with o-phthaldeyde in the presence of N-acetyl-L-cysteine.
"Enantiomeric resolution of amino acids by reversed phase high performance liquid chromatography using a new mobile phase" / Federici, F.; Girelli, Anna Maria; Messina, Antonella; Nicoletti, I.; Sinibaldi, M.. - In: ANALYTICAL LETTERS. - ISSN 0003-2719. - STAMPA. - 23:(1990), pp. 1565-1579. [10.1080/00032719008052508]