Adventitious shoot regeneration was obtained from callus produced from main vegetative apices of pear of in vitro grown shoots of Italian cultivars Spadona and Precoce di Fiorano and wild pear genotypes ISF54 and ISF61. The highest morphogenetic response was obtained on a medium containing 8.8 muM 6-benzyladenine, 1.0 muM alpha-naphthaleneacetic acid and 250 mg l(-)1cefotaxime. The explants were maintained for 30 days in darkness and then transferred to an auxin-free medium and to the light. Histological studies revealed that the new vegetative buds originated from callus that completely altered the morphology of the explant tissues by the 30th day of culture. The in situ localisation of cytokinins, performed using antibodies with marked specificity against zeatin (Z) and isopentenyladenine, revealed an accumulation of Z in the cambiform cells of the leaf primordia and in the shell zone of the new forming buds showing a primary role of this cytokinin in cell differentiation of in vitro pear organogenesis.

Adventitious shoot regeneration from vegetative shoot apices in pear and putative role of cytokinin accumulation in the morphogenetic process / E., Caboni; P., Lauri; D'Angeli, Simone; A., Chiappetta; A. M., Innocenti; H., Van Onckelen; C., Damiano. - In: PLANT CELL TISSUE AND ORGAN CULTURE. - ISSN 0167-6857. - STAMPA. - 70:2(2002), pp. 199-206. (Intervento presentato al convegno 1st Meeting of Working Group 1 of COST action 843 tenutosi a GEISENHEIM, GERMANY nel OCT 12-15, 2000) [10.1023/a:1016304106529].

Adventitious shoot regeneration from vegetative shoot apices in pear and putative role of cytokinin accumulation in the morphogenetic process

D'ANGELI, Simone;
2002

Abstract

Adventitious shoot regeneration was obtained from callus produced from main vegetative apices of pear of in vitro grown shoots of Italian cultivars Spadona and Precoce di Fiorano and wild pear genotypes ISF54 and ISF61. The highest morphogenetic response was obtained on a medium containing 8.8 muM 6-benzyladenine, 1.0 muM alpha-naphthaleneacetic acid and 250 mg l(-)1cefotaxime. The explants were maintained for 30 days in darkness and then transferred to an auxin-free medium and to the light. Histological studies revealed that the new vegetative buds originated from callus that completely altered the morphology of the explant tissues by the 30th day of culture. The in situ localisation of cytokinins, performed using antibodies with marked specificity against zeatin (Z) and isopentenyladenine, revealed an accumulation of Z in the cambiform cells of the leaf primordia and in the shell zone of the new forming buds showing a primary role of this cytokinin in cell differentiation of in vitro pear organogenesis.
2002
1st Meeting of Working Group 1 of COST action 843
immunolocalization; histological analysis; callus; zeatin; isopentenyladenine; plant regeneration; pyrus communis l.; tissue culture
04 Pubblicazione in atti di convegno::04c Atto di convegno in rivista
Adventitious shoot regeneration from vegetative shoot apices in pear and putative role of cytokinin accumulation in the morphogenetic process / E., Caboni; P., Lauri; D'Angeli, Simone; A., Chiappetta; A. M., Innocenti; H., Van Onckelen; C., Damiano. - In: PLANT CELL TISSUE AND ORGAN CULTURE. - ISSN 0167-6857. - STAMPA. - 70:2(2002), pp. 199-206. (Intervento presentato al convegno 1st Meeting of Working Group 1 of COST action 843 tenutosi a GEISENHEIM, GERMANY nel OCT 12-15, 2000) [10.1023/a:1016304106529].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/346474
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