A kinetic study of ornithine carbamyl transferase (OCT) from Streptococcus faecalis has been carried out, employing a new HPLC method based on the direct determination of citrulline. A different kinetic pattern was observed in tris(hydroxymethyl)-aminomethane (TRIS) and triethanolamine (TEA) buffers: a ping-pong or an ordered sequential mechanism are suggested, respectively. The inhibition of Tris buffer on OCT reaction has also been demonstrated. The inhibition of Tris buffer on OCT reaction has also been demonstrated.
"Buffer effect on the kinetics of ornithine carbamyl transferase by HPLC" / Carunchio, Vincenzo Tito; Girelli, Anna Maria; Messina, Antonella. - In: BIOMEDICAL CHROMATOGRAPHY. - ISSN 0269-3879. - STAMPA. - 13:(1999), pp. 65-69. [10.1002/(SICI)1099-0801(199902)13:1]
"Buffer effect on the kinetics of ornithine carbamyl transferase by HPLC"
CARUNCHIO, Vincenzo Tito;GIRELLI, Anna Maria;MESSINA, Antonella
1999
Abstract
A kinetic study of ornithine carbamyl transferase (OCT) from Streptococcus faecalis has been carried out, employing a new HPLC method based on the direct determination of citrulline. A different kinetic pattern was observed in tris(hydroxymethyl)-aminomethane (TRIS) and triethanolamine (TEA) buffers: a ping-pong or an ordered sequential mechanism are suggested, respectively. The inhibition of Tris buffer on OCT reaction has also been demonstrated. The inhibition of Tris buffer on OCT reaction has also been demonstrated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
