Three chiral stationary phases, obtained by grafting silica gel with (-)-trans-1,2-cyclohexanediamine, were studied for the resolution of -amino acids by ligand-exchange chromatography. The packings were prepared by bonding the chiral ligand to silica gel via different hydrocarbon spacers. Separation of the optical isomers was accomplished by eluents containing a constant concentration of copper(II) acetate (0.05mM). The elution sequence of amino acids was found to be dependent on the grafting reaction selected to prepare the chiral packings.
"High-performance ligand-exchange chromatography of amino acids on chiral stationary phases" / Carunchio, Vincenzo Tito; Messina, Antonella; Sinibaldi, M.; Fanali, S.. - In: HRC- JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY. - ISSN 0935-6304. - STAMPA. - 11:(1988), pp. 401-404. [10.1002/jhrc.1240110508]
"High-performance ligand-exchange chromatography of amino acids on chiral stationary phases"
CARUNCHIO, Vincenzo Tito;MESSINA, Antonella;
1988
Abstract
Three chiral stationary phases, obtained by grafting silica gel with (-)-trans-1,2-cyclohexanediamine, were studied for the resolution of -amino acids by ligand-exchange chromatography. The packings were prepared by bonding the chiral ligand to silica gel via different hydrocarbon spacers. Separation of the optical isomers was accomplished by eluents containing a constant concentration of copper(II) acetate (0.05mM). The elution sequence of amino acids was found to be dependent on the grafting reaction selected to prepare the chiral packings.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
