We describe the nonenzymatic ligation of RNA oligomers in water. Dimers and tetramers are formed in a time-, pH-, and temperature-dependent reaction. Ligation efficiency depends on oligonucleotide length and sequence and is strongly enhanced by adenine-based nucleotide cofactors. Ligation of short RNA fragments could have liberated the prebiotic polymerization systems from the thermodynamically demanding task of reaching a (pre) genetically meaningful size by stepwise addition of one precursor monomer at the time.

Nonenzymatic RNA Ligation in Water / Pino, Samanta; Ciciriello, Fabiana; Giovanna Maria, Costanzo; DI MAURO, Ernesto. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - STAMPA. - 283:52(2008), pp. 36494-36503. [10.1074/jbc.m805333200]

Nonenzymatic RNA Ligation in Water

PINO, SAMANTA;CICIRIELLO, FABIANA;DI MAURO, Ernesto
2008

Abstract

We describe the nonenzymatic ligation of RNA oligomers in water. Dimers and tetramers are formed in a time-, pH-, and temperature-dependent reaction. Ligation efficiency depends on oligonucleotide length and sequence and is strongly enhanced by adenine-based nucleotide cofactors. Ligation of short RNA fragments could have liberated the prebiotic polymerization systems from the thermodynamically demanding task of reaching a (pre) genetically meaningful size by stepwise addition of one precursor monomer at the time.
2008
01 Pubblicazione su rivista::01a Articolo in rivista
Nonenzymatic RNA Ligation in Water / Pino, Samanta; Ciciriello, Fabiana; Giovanna Maria, Costanzo; DI MAURO, Ernesto. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - STAMPA. - 283:52(2008), pp. 36494-36503. [10.1074/jbc.m805333200]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/31839
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