We describe the nonenzymatic ligation of RNA oligomers in water. Dimers and tetramers are formed in a time-, pH-, and temperature-dependent reaction. Ligation efficiency depends on oligonucleotide length and sequence and is strongly enhanced by adenine-based nucleotide cofactors. Ligation of short RNA fragments could have liberated the prebiotic polymerization systems from the thermodynamically demanding task of reaching a (pre) genetically meaningful size by stepwise addition of one precursor monomer at the time.
Nonenzymatic RNA Ligation in Water / Pino, Samanta; Ciciriello, Fabiana; Giovanna Maria, Costanzo; DI MAURO, Ernesto. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - STAMPA. - 283:52(2008), pp. 36494-36503. [10.1074/jbc.m805333200]
Nonenzymatic RNA Ligation in Water
PINO, SAMANTA;CICIRIELLO, FABIANA;DI MAURO, Ernesto
2008
Abstract
We describe the nonenzymatic ligation of RNA oligomers in water. Dimers and tetramers are formed in a time-, pH-, and temperature-dependent reaction. Ligation efficiency depends on oligonucleotide length and sequence and is strongly enhanced by adenine-based nucleotide cofactors. Ligation of short RNA fragments could have liberated the prebiotic polymerization systems from the thermodynamically demanding task of reaching a (pre) genetically meaningful size by stepwise addition of one precursor monomer at the time.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
