Dynamic alterations in chromatin structure mediated by postsynthetic histone modifications and DNA methylation constitute a major regulatory mechanism in DNA functioning. DNA methylation has been implicated in transcriptional silencing, in part by inducing chromatin condensation. To understand the methylation-dependent chromatin structure, we performed atomic force microscope (AFM) studies of fibers isolated from cultured cells containing normal or elevated levels of m(5)C. Chromatin fibers were reconstituted on control or methylated DNA templates in the presence or absence of linker histone. Visual inspection of AFM images, combined with quantitative analysis of fiber structural parameters, suggested that DNA methylation induced fiber compaction only in the presence of linker histones. This conclusion was further substantiated by biochemical results.

DNA methylation-dependent chromatin fiber compaction in vivo and in vitro: requirement for linker histone / M. A., Karimov; M., Tomschik; S. H., Leuba; Caiafa, Paola; J., Zlatanova. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - STAMPA. - 15:14(2001), pp. 2631-2641. [10.1096/fj.01-0345com]

DNA methylation-dependent chromatin fiber compaction in vivo and in vitro: requirement for linker histone

CAIAFA, Paola;
2001

Abstract

Dynamic alterations in chromatin structure mediated by postsynthetic histone modifications and DNA methylation constitute a major regulatory mechanism in DNA functioning. DNA methylation has been implicated in transcriptional silencing, in part by inducing chromatin condensation. To understand the methylation-dependent chromatin structure, we performed atomic force microscope (AFM) studies of fibers isolated from cultured cells containing normal or elevated levels of m(5)C. Chromatin fibers were reconstituted on control or methylated DNA templates in the presence or absence of linker histone. Visual inspection of AFM images, combined with quantitative analysis of fiber structural parameters, suggested that DNA methylation induced fiber compaction only in the presence of linker histones. This conclusion was further substantiated by biochemical results.
2001
atomic force microscopy; chromatin fibers; chromatin structure; dna methylation; histone h1
01 Pubblicazione su rivista::01a Articolo in rivista
DNA methylation-dependent chromatin fiber compaction in vivo and in vitro: requirement for linker histone / M. A., Karimov; M., Tomschik; S. H., Leuba; Caiafa, Paola; J., Zlatanova. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - STAMPA. - 15:14(2001), pp. 2631-2641. [10.1096/fj.01-0345com]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/31566
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