We investigated, for the first time, by using Energy Dispersive X-ray Diffraction, the structure of a new ternary cationic liposome formulated with dioleoyl trimethylammonium propane (DOTAP), 1,2-dioleoyl-3-phosphatidylethanolamine (DOPE) and cholesterol (Chol) (DDC) which has been recently found to have a selective high gene transfer ability in ovarian cancer cells. Our structural results provide a further experimental support to the widely accepted statement that there is not a simple and direct correlation between structure and transfection efficiency and that the factors controlling cationic lipid/DNA (CL-DNA) complexes-mediated gene transfer depend not only on the formulations of the cationic liposomes and their thermodynamic phase, but also significantly on the cell properties. Document Type: Article
Structural characterization of a new lipid/DNA complex showing a selective transfection efficiency in ovarian cancer cells / Caracciolo, Giulio; Pozzi, Daniela; Caminiti, Ruggero; Congiu, Agostina. - In: THE EUROPEAN PHYSICAL JOURNAL. E, SOFT MATTER. - ISSN 1292-8941. - STAMPA. - 10:4(2003), pp. 331-336. [10.1140/epje/i2002-10117-x]
Structural characterization of a new lipid/DNA complex showing a selective transfection efficiency in ovarian cancer cells
CARACCIOLO, Giulio;POZZI, DANIELA;CAMINITI, Ruggero;CONGIU, Agostina
2003
Abstract
We investigated, for the first time, by using Energy Dispersive X-ray Diffraction, the structure of a new ternary cationic liposome formulated with dioleoyl trimethylammonium propane (DOTAP), 1,2-dioleoyl-3-phosphatidylethanolamine (DOPE) and cholesterol (Chol) (DDC) which has been recently found to have a selective high gene transfer ability in ovarian cancer cells. Our structural results provide a further experimental support to the widely accepted statement that there is not a simple and direct correlation between structure and transfection efficiency and that the factors controlling cationic lipid/DNA (CL-DNA) complexes-mediated gene transfer depend not only on the formulations of the cationic liposomes and their thermodynamic phase, but also significantly on the cell properties. Document Type: ArticleI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.