A mixture of dimers of nicotinamide adenine dinucleotide, largely 4,4--linked, obtained by electrochemical reduction of NAD+, can be photooxidized back to NAD+ in the presence of oxygen. Oxygen is consumed during the photooxidation process with the production of hydrogen peroxide. The oxidation is almost pH independent and is stimulated by light whose wavelength exceeds 300 nm. Lactate dehydrogenase and alcohol dehydrogenase added to the solutions under irradiation increased the oxygen uptake by the NAD dimers in a concentration-dependent way. These observations suggest that light induces the homolytic cleavage of NAD dimers to NAD radicals which in turn are oxidized to NAD+ by oxygen
photochemical oxidation of NAD dimers / Avigliano, L; Carelli, Vincenzo; Casini, Antonio; FINAZZI AGRÒ, A.; Liberatore, Felice. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - STAMPA. - 723:(1983), pp. 372-375.
photochemical oxidation of NAD dimers
CARELLI, Vincenzo;CASINI, Antonio;LIBERATORE, Felice
1983
Abstract
A mixture of dimers of nicotinamide adenine dinucleotide, largely 4,4--linked, obtained by electrochemical reduction of NAD+, can be photooxidized back to NAD+ in the presence of oxygen. Oxygen is consumed during the photooxidation process with the production of hydrogen peroxide. The oxidation is almost pH independent and is stimulated by light whose wavelength exceeds 300 nm. Lactate dehydrogenase and alcohol dehydrogenase added to the solutions under irradiation increased the oxygen uptake by the NAD dimers in a concentration-dependent way. These observations suggest that light induces the homolytic cleavage of NAD dimers to NAD radicals which in turn are oxidized to NAD+ by oxygenI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
