IgG antibodies containing anti-IgE activity isolated from a patient with atopic dermatitis (H-aIgE) induced mediator release from human basophils and mast cells isolated from skin and lung tissues. The release of histamine was calcium- and temperature-dependent and did not involve cytotoxicity. There was an excellent correlation (r = 0.88; p less than 0.001) between the maximum percent histamine release from human basophils induced by rabbit anti-IgE (R-aIgE) and H-aIgE. H-aIgE was approximately 30 times more potent than R-aIgE in inducing mediator release from human basophils, skin, and lung mast cells. H-aIgE specifically desensitized human basophils to a subsequent challenge with both H-aIgE and R-aIgE. Lactic acid removal of IgE from human basophils blocked the releasing activity of both R-aIgE and H-aIgE. Passive sensitization with hyperimmune sera or purified IgE myeloma restored the response of basophils to both R-aIgE and H-aIgE. IgE purified from three different myeloma patients concentration-dependently blocked the histamine releasing activity of both R-aIgE and H-aIgE.

IGG ANTI-IGE FROM ATOPIC-DERMATITIS INDUCES MEDIATOR RELEASE FROM BASOPHILS AND MAST-CELLS / Marone, G; Casolaro, V; Paganelli, R; Quinti, Isabella. - In: JOURNAL OF INVESTIGATIVE DERMATOLOGY. - ISSN 0022-202X. - STAMPA. - 93:(1989), pp. 246-252. [10.1111/1523-1747.ep12277582]

IGG ANTI-IGE FROM ATOPIC-DERMATITIS INDUCES MEDIATOR RELEASE FROM BASOPHILS AND MAST-CELLS

QUINTI, Isabella
1989

Abstract

IgG antibodies containing anti-IgE activity isolated from a patient with atopic dermatitis (H-aIgE) induced mediator release from human basophils and mast cells isolated from skin and lung tissues. The release of histamine was calcium- and temperature-dependent and did not involve cytotoxicity. There was an excellent correlation (r = 0.88; p less than 0.001) between the maximum percent histamine release from human basophils induced by rabbit anti-IgE (R-aIgE) and H-aIgE. H-aIgE was approximately 30 times more potent than R-aIgE in inducing mediator release from human basophils, skin, and lung mast cells. H-aIgE specifically desensitized human basophils to a subsequent challenge with both H-aIgE and R-aIgE. Lactic acid removal of IgE from human basophils blocked the releasing activity of both R-aIgE and H-aIgE. Passive sensitization with hyperimmune sera or purified IgE myeloma restored the response of basophils to both R-aIgE and H-aIgE. IgE purified from three different myeloma patients concentration-dependently blocked the histamine releasing activity of both R-aIgE and H-aIgE.
1989
01 Pubblicazione su rivista::01a Articolo in rivista
IGG ANTI-IGE FROM ATOPIC-DERMATITIS INDUCES MEDIATOR RELEASE FROM BASOPHILS AND MAST-CELLS / Marone, G; Casolaro, V; Paganelli, R; Quinti, Isabella. - In: JOURNAL OF INVESTIGATIVE DERMATOLOGY. - ISSN 0022-202X. - STAMPA. - 93:(1989), pp. 246-252. [10.1111/1523-1747.ep12277582]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/257287
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