Purified adult rat Leydig cells were found to produce gastrin-releasing peptide (GRP) by radioimmunoassay (RIA). Gel chromatography of the extracted material showed a single peak of GRP immunoreactivity. Further high pressure liquid chromatography (HPLC) analysis resolved the extract into two peaks that closely resembled the C-terminal fragment of GRP, GRP18-27 and GRP14-27. Immunohistochemical studies revealed specific staining for GRP in the Leydig cells of adult rat testis. These results demonstrate, by a number of independent criteria, that rat Leydig cells contain substances which behave like authentic GRP-like peptides. Since the peptides appear to be of local origin, a paracrine function within the rat testis is suggested.
Identification of immunoreactive Gastrin Releasing Peptide related substances in adult rat Leydig cell / Gnessi, Lucio; Isidori, A; Bolotti, M; Altamura, S; Ulisse, Salvatore; Jannini, Ea; Fabbri, A; Spera, G.. - In: ENDOCRINOLOGY. - ISSN 0013-7227. - STAMPA. - 124:(1989), pp. 558-560. [10.1210/endo-124-1-558]
Identification of immunoreactive Gastrin Releasing Peptide related substances in adult rat Leydig cell.
GNESSI, Lucio;ULISSE, SALVATORE;
1989
Abstract
Purified adult rat Leydig cells were found to produce gastrin-releasing peptide (GRP) by radioimmunoassay (RIA). Gel chromatography of the extracted material showed a single peak of GRP immunoreactivity. Further high pressure liquid chromatography (HPLC) analysis resolved the extract into two peaks that closely resembled the C-terminal fragment of GRP, GRP18-27 and GRP14-27. Immunohistochemical studies revealed specific staining for GRP in the Leydig cells of adult rat testis. These results demonstrate, by a number of independent criteria, that rat Leydig cells contain substances which behave like authentic GRP-like peptides. Since the peptides appear to be of local origin, a paracrine function within the rat testis is suggested.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
