We have demonstrated that the high resistance to imidazoles in 60 Escherichia coli strains is overcome by lowering the minimum inhibitory concentration (MIC) in culture from >3200 to <0.39 mu g ml(-1) and, by determining, in contact tests, the disappearance of 10(4) CFU with 25 mu g ml(-1) of imidazoles after 3 min. Although the contact. activity was not the same as growth inhibition due to the different target of the drug in the cell structures, we annulled E. coli imidazole resistance in cultural and direct tests by means of the same non-antimicrobial conditions related to outer membrane functions.
Overcoming imidazole resistance in Escherichia coli / Simonetti, Giovanna; S., Baffa; Simonetti, Nicola. - In: JOURNAL OF CHEMOTHERAPY. - ISSN 1120-009X. - 8:3(1996), pp. 200-206.
Overcoming imidazole resistance in Escherichia coli
SIMONETTI, Giovanna;SIMONETTI, Nicola
1996
Abstract
We have demonstrated that the high resistance to imidazoles in 60 Escherichia coli strains is overcome by lowering the minimum inhibitory concentration (MIC) in culture from >3200 to <0.39 mu g ml(-1) and, by determining, in contact tests, the disappearance of 10(4) CFU with 25 mu g ml(-1) of imidazoles after 3 min. Although the contact. activity was not the same as growth inhibition due to the different target of the drug in the cell structures, we annulled E. coli imidazole resistance in cultural and direct tests by means of the same non-antimicrobial conditions related to outer membrane functions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.