We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROalpha by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP3) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROalpha may play a neuromodulatory role on mouse cerebellum.

CXC chemokine interleukin-8 (IL-8) and growth-related gene product (GROalpha)modulate Purkinje neuron activity in mouse cerebellum / Giovannelli, A.; Limatola, Cristina; Ragozzino, Davide Antonio; Mileo, A. M.; Ruggieri, A.; Ciotti, M. T.; Mercanti, D.; Santoni, Angela; Eusebi, Fabrizio. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - STAMPA. - 92:(1998), pp. 122-132. [10.1016/S0165-5728(98)00192-1]

CXC chemokine interleukin-8 (IL-8) and growth-related gene product (GROalpha)modulate Purkinje neuron activity in mouse cerebellum

LIMATOLA, Cristina;RAGOZZINO, Davide Antonio;SANTONI, Angela;EUSEBI, Fabrizio
1998

Abstract

We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROalpha by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP3) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROalpha may play a neuromodulatory role on mouse cerebellum.
1998
01 Pubblicazione su rivista::01a Articolo in rivista
CXC chemokine interleukin-8 (IL-8) and growth-related gene product (GROalpha)modulate Purkinje neuron activity in mouse cerebellum / Giovannelli, A.; Limatola, Cristina; Ragozzino, Davide Antonio; Mileo, A. M.; Ruggieri, A.; Ciotti, M. T.; Mercanti, D.; Santoni, Angela; Eusebi, Fabrizio. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - STAMPA. - 92:(1998), pp. 122-132. [10.1016/S0165-5728(98)00192-1]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/256923
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