OBJECTIVES: To develop an enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody (mab) directed against abnormally glycosylated serum alpha2-macroglobulin (alpha2-M) from patients with systemic lupus erythematosus (SLE). DESIGN AND METHODS: Serum alpha2-M purified by HPLC from patients with SLE was injected in a Balb/c, CB6 F1 female mouse and hybrid cell lines were screened using alpha2-M Glu-C fragments derived from SLE and normal donors (NHS). A mab was selected and used to develop an ELISA by which sera from NHS (n = 14), SLE (n = 34), rheumatoid arthritis (n = 15), Sjögren's syndrome (n = 11), mixed connective tissue diseases (n = 12), and liver diseases (n = 11) were analyzed. RESULTS: The affinity of the mab for alpha2-M from SLE, but not from the other diseases, was higher compared to NHS, as demonstrated by immunoblotting and ELISA. CONCLUSIONS: The ELISA was capable of recognizing changes of glycosylation of alpha2-M in SLE and may be useful for its differential diagnosis.

Development of an enzyme-linked immunosorbent assay, using a monoclonal antibody against a2-macroglobulin, for the diagnosis of systemic lupus erythematosus / Cazzolla, N.; Saso, Luciano; Grima, J.; Leone, M. G.; Grippa, E.; Cheng, C. Y.; Silvestrini, Bruno. - In: CLINICAL BIOCHEMISTRY. - ISSN 0009-9120. - 32: n. 4:(1999), pp. 249-255. [10.1016/S0009-9120(99)00010-7]

Development of an enzyme-linked immunosorbent assay, using a monoclonal antibody against a2-macroglobulin, for the diagnosis of systemic lupus erythematosus

SASO, Luciano;SILVESTRINI, Bruno
1999

Abstract

OBJECTIVES: To develop an enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody (mab) directed against abnormally glycosylated serum alpha2-macroglobulin (alpha2-M) from patients with systemic lupus erythematosus (SLE). DESIGN AND METHODS: Serum alpha2-M purified by HPLC from patients with SLE was injected in a Balb/c, CB6 F1 female mouse and hybrid cell lines were screened using alpha2-M Glu-C fragments derived from SLE and normal donors (NHS). A mab was selected and used to develop an ELISA by which sera from NHS (n = 14), SLE (n = 34), rheumatoid arthritis (n = 15), Sjögren's syndrome (n = 11), mixed connective tissue diseases (n = 12), and liver diseases (n = 11) were analyzed. RESULTS: The affinity of the mab for alpha2-M from SLE, but not from the other diseases, was higher compared to NHS, as demonstrated by immunoblotting and ELISA. CONCLUSIONS: The ELISA was capable of recognizing changes of glycosylation of alpha2-M in SLE and may be useful for its differential diagnosis.
1999
enzyme-linked immunosorbent assay; monoclonal antibody alpha2-macroglobulin; systemic lupus erythematosus
01 Pubblicazione su rivista::01a Articolo in rivista
Development of an enzyme-linked immunosorbent assay, using a monoclonal antibody against a2-macroglobulin, for the diagnosis of systemic lupus erythematosus / Cazzolla, N.; Saso, Luciano; Grima, J.; Leone, M. G.; Grippa, E.; Cheng, C. Y.; Silvestrini, Bruno. - In: CLINICAL BIOCHEMISTRY. - ISSN 0009-9120. - 32: n. 4:(1999), pp. 249-255. [10.1016/S0009-9120(99)00010-7]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/256706
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