Purpose. To analyze the pharmacologic profile and the anatomic localization of the dopamine D 1 and D 2 receptors in sections of the rabbit cornea in normal conditions. Methods. Samples of rabbit cornea were drawn. Biochemical and autoradiographic techniques were used on frozen sections. [ 3H]SCH-23390 was used as a ligand of dopamine D 1 receptors and [ 3H]spiroperidol as a ligand of dopamine D 2 receptors. Results. [ 3H]SCH- 23390 and [ 3H]spiroperidol were bound by sections of the rabbit cornea. The pharmacologic profile of the binding was consistent with the labeling of D 1 and D 2 receptors, respectively. Light microscopic analysis of the localization of D 1 and D 2 receptors revealed an accumulation of the two radioligands in the epithelial and in the endothelial layers of the cornea. Conclusion. A possible role of the dopaminergic system in the control of the corneal functions is suggested.
Localization of Dopamine receptors in rabbit cornea / Cavallotti, Carlo; Pescosolido, Nicola; Artico, Marco; Feher, J.. - In: CORNEA. - ISSN 0277-3740. - STAMPA. - 18:6(1999), pp. 721-728. [10.1097/00003226-199911000-00016]
Localization of Dopamine receptors in rabbit cornea
CAVALLOTTI, Carlo;PESCOSOLIDO, Nicola;ARTICO, Marco;
1999
Abstract
Purpose. To analyze the pharmacologic profile and the anatomic localization of the dopamine D 1 and D 2 receptors in sections of the rabbit cornea in normal conditions. Methods. Samples of rabbit cornea were drawn. Biochemical and autoradiographic techniques were used on frozen sections. [ 3H]SCH-23390 was used as a ligand of dopamine D 1 receptors and [ 3H]spiroperidol as a ligand of dopamine D 2 receptors. Results. [ 3H]SCH- 23390 and [ 3H]spiroperidol were bound by sections of the rabbit cornea. The pharmacologic profile of the binding was consistent with the labeling of D 1 and D 2 receptors, respectively. Light microscopic analysis of the localization of D 1 and D 2 receptors revealed an accumulation of the two radioligands in the epithelial and in the endothelial layers of the cornea. Conclusion. A possible role of the dopaminergic system in the control of the corneal functions is suggested.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
