PREVIOUS experiments have shown that human neoplastic and embryonic glial cell lines synthesize and secrete in culture, alpha2macroglobulin (alpha2M), a broad spectrum proteinase inhibitor present in serum and extracellular fluids. The present study -as aimed to investigate the presence of alpha2M receptors on glial cell membrane, since several non-neural cell types producing alpha2M also express alpha2M receptors. By flow cytometric analysis, immunofluorescence and immunoelectronmicroscopy techniques we demonstrate an alpha2M receptor-related immunoreactivity on the plasma membrane of a human glioma cell line. Ultrastructural experiments reveal a close colocalization of immunoreactivities for alpha2M and its receptor in clathrin-coated pits and vesicles, structures typically involved in receptor-mediated endocytic pathways.
Receptor-mediated endocytosis of alpha 2 macroglobulin by a glioma cell line / S. L., Nori; Businaro, Rita; Fabrizi, Cinzia; S. K., Moestrup; G., Starace; Fumagalli, Lorenzo; G. M., Lauro. - In: NEUROREPORT. - ISSN 0959-4965. - 4:4(1993), pp. 423-426. [10.1097/00001756-199304000-00021]
Receptor-mediated endocytosis of alpha 2 macroglobulin by a glioma cell line.
BUSINARO, Rita;FABRIZI, CINZIA;FUMAGALLI, Lorenzo;
1993
Abstract
PREVIOUS experiments have shown that human neoplastic and embryonic glial cell lines synthesize and secrete in culture, alpha2macroglobulin (alpha2M), a broad spectrum proteinase inhibitor present in serum and extracellular fluids. The present study -as aimed to investigate the presence of alpha2M receptors on glial cell membrane, since several non-neural cell types producing alpha2M also express alpha2M receptors. By flow cytometric analysis, immunofluorescence and immunoelectronmicroscopy techniques we demonstrate an alpha2M receptor-related immunoreactivity on the plasma membrane of a human glioma cell line. Ultrastructural experiments reveal a close colocalization of immunoreactivities for alpha2M and its receptor in clathrin-coated pits and vesicles, structures typically involved in receptor-mediated endocytic pathways.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
