Gas-phase hydrogen/deuterium exchange reactions of (de)protonated (sodiated) adenosine-5'-mono-, di- and triphosphate ions with CD3OD, CD3CO2D and ND3 were achieved using a combination of electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry. The reaction kinetics are dependent on factors such as the charge state, the phosphate chain length, the properties of the exchange reactants and the sodium content. The results indicate that the overall H/D exchange may involve specific sites even if endowed with high energetic barriers. The enhanced reactivity exhibited by adenosine polyphosphate ions compared with adenosine-5'-monophosphate suggests a critical role of the polyphosphate chain in rendering conformationally accessible remote H-donor sites. Low-energy collision-induced dissociation of (sodiated) adenine nucleotides anions supports the aptitude of the (poly)phosphate chain in probing distant sites via the intermediacy of a cyclic structure. Copyright (C) 2003 John Wiley Sons, Ltd.

Gas-phase hydrogen/deuterium exchange of adenine nucleotides / Crestoni, Maria Elisa; Fornarini, Simonetta. - In: JOURNAL OF MASS SPECTROMETRY. - ISSN 1076-5174. - STAMPA. - 38:8(2003), pp. 854-861. [10.1002/jms.498]

Gas-phase hydrogen/deuterium exchange of adenine nucleotides

CRESTONI, Maria Elisa;FORNARINI, Simonetta
2003

Abstract

Gas-phase hydrogen/deuterium exchange reactions of (de)protonated (sodiated) adenosine-5'-mono-, di- and triphosphate ions with CD3OD, CD3CO2D and ND3 were achieved using a combination of electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry. The reaction kinetics are dependent on factors such as the charge state, the phosphate chain length, the properties of the exchange reactants and the sodium content. The results indicate that the overall H/D exchange may involve specific sites even if endowed with high energetic barriers. The enhanced reactivity exhibited by adenosine polyphosphate ions compared with adenosine-5'-monophosphate suggests a critical role of the polyphosphate chain in rendering conformationally accessible remote H-donor sites. Low-energy collision-induced dissociation of (sodiated) adenine nucleotides anions supports the aptitude of the (poly)phosphate chain in probing distant sites via the intermediacy of a cyclic structure. Copyright (C) 2003 John Wiley Sons, Ltd.
2003
conformational isomers; electrospray ionization; fourier transform ion cyclotron resonance mass; fourier transform ion cyclotron resonance mass spectrometry; gas phase kinetics; h/d exchange; ion-molecule reaction; nucleotide; spectrometry; structure elucidation
01 Pubblicazione su rivista::01a Articolo in rivista
Gas-phase hydrogen/deuterium exchange of adenine nucleotides / Crestoni, Maria Elisa; Fornarini, Simonetta. - In: JOURNAL OF MASS SPECTROMETRY. - ISSN 1076-5174. - STAMPA. - 38:8(2003), pp. 854-861. [10.1002/jms.498]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/256403
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