The morphological pattern of several enzymes (succinic dehydrogenase - SDH, glucose-6-phosphate dehydrogenase - G6PDH and lactic dehydrogenase - LDH) was evaluated in normal dog eyes. Special attention was paid to the uveo-scleral tissue. Cryostatic sections of dog eye were stained with toluidine blue for the recognition of the microanatomical details or with histoenzymatic methods for SDH, G6PDH and LDH activities using sodium succinate, glucose-6-phosphate and sodium lactate as substrates respectively, nicotinamide adenine dinucleotide (NAD) as a reducing agent and sodium nitro-blue-tetrazolium as a colouring substance. A moderate positive reaction for SDH and a strong positive reaction for LDH were observed in the uveo-scleral tissue, while G6PDH gave negative staining. Some considerations regarding a possible active role of these enzymatic activities to the aqueous humor outflow are suggested
Uveoscleral outflowin dog's eye: role of several enzymes / Cavallotti, Carlo; Pescosolido, Nicola; Artico, M.; Cavallotti, Carlo. - In: INTERNATIONAL OPHTHALMOLOGY. - ISSN 0165-5701. - STAMPA. - 22:4(1999), pp. 233-238. [10.1023/A:1006332713984]
Uveoscleral outflowin dog's eye: role of several enzymes.
CAVALLOTTI, Carlo;PESCOSOLIDO, Nicola;M. Artico;CAVALLOTTI, Carlo
1999
Abstract
The morphological pattern of several enzymes (succinic dehydrogenase - SDH, glucose-6-phosphate dehydrogenase - G6PDH and lactic dehydrogenase - LDH) was evaluated in normal dog eyes. Special attention was paid to the uveo-scleral tissue. Cryostatic sections of dog eye were stained with toluidine blue for the recognition of the microanatomical details or with histoenzymatic methods for SDH, G6PDH and LDH activities using sodium succinate, glucose-6-phosphate and sodium lactate as substrates respectively, nicotinamide adenine dinucleotide (NAD) as a reducing agent and sodium nitro-blue-tetrazolium as a colouring substance. A moderate positive reaction for SDH and a strong positive reaction for LDH were observed in the uveo-scleral tissue, while G6PDH gave negative staining. Some considerations regarding a possible active role of these enzymatic activities to the aqueous humor outflow are suggestedI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
