Nucleosome positioning along two DNA tracts, corresponding to tetramers of the light-responsive elements of pea rbcS-3A and rbcS-3.6 genes, were studied by experimental (exonuclease III mapping and band shift electrophoresis) as well as theoretical methods. Multiple nucleosome positioning with unique rotational phase was derived from both methods in satisfactorily good agreement, if nucleosome dyad axis positions are considered. Theoretical and experimental distributions of nucleosome frequencies appear different, probably on account of DNA sequence dependent digestion kinetics of exonuclease III.
Multiple nucleosome positioning with unique rotational phasing on multimers of the Light Responsive Elements of pea rbcS-3A and rbcS-3.6 genes: comparison between experimental and theoretical mapping / R., Alessi; Cacchione, Stefano; DE SANTIS, Pasquale; M., Fua'; Savino, Maria. - In: BIOPHYSICAL CHEMISTRY. - ISSN 0301-4622. - STAMPA. - 67:(1997), pp. 151-158. [10.1016/S0301-4622(97)00035-5]
Multiple nucleosome positioning with unique rotational phasing on multimers of the Light Responsive Elements of pea rbcS-3A and rbcS-3.6 genes: comparison between experimental and theoretical mapping.
CACCHIONE, Stefano;DE SANTIS, Pasquale;SAVINO, Maria
1997
Abstract
Nucleosome positioning along two DNA tracts, corresponding to tetramers of the light-responsive elements of pea rbcS-3A and rbcS-3.6 genes, were studied by experimental (exonuclease III mapping and band shift electrophoresis) as well as theoretical methods. Multiple nucleosome positioning with unique rotational phase was derived from both methods in satisfactorily good agreement, if nucleosome dyad axis positions are considered. Theoretical and experimental distributions of nucleosome frequencies appear different, probably on account of DNA sequence dependent digestion kinetics of exonuclease III.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
