The separation of some anthrone-C-glucosyl constituents of aloe was carried out by isocratic capillary electrochromatography using a 50 I~m I. D. column packed with 3 I~m ODS silica gel. In comparison to HPLC mode, CEC resolution of aloins was obtained in a very short time with a high selectivi b, factor. The influence of mobile phase parameters (pH and ionic strength of the buffer, acetonitrile amount in the eluent etc.) on the retention of aloins was examined in order to improve the separation conditions and to study the retention mechanism. Efficiency was found to increase with decreasing the EOF up to a reduced plate height of about 2 at a reduced linear velocif/of 0.3 mm s 1. This effect was ascribed to the fact that, under the buffer conditions used (pH 8), aloins are partially anionically charged, with a consequent low mass transfer bel',,veen the mobile and stationary phases. However, resolution remains almost the same in a wide pH range permitting baseline separations of aloins in short analysis times. Since aloins are known to undergo fast degradation reaction dependent on pH, a kinetic study was also carried out. The method was extended to the analysis of aloins in food and cosmetic products.

Reversed-phase capillary electrochromatography of aloins and related constituents of aloe / Girelli, Anna Maria; Messina, Antonella; Ferrantelli, P.; Sinibaldi, M.; Tarola, Anna Maria. - In: CHROMATOGRAPHIA. - ISSN 0009-5893. - ELETTRONICO. - 53:(2001), pp. 284-289. [10.1007/BF02490343]

Reversed-phase capillary electrochromatography of aloins and related constituents of aloe

GIRELLI, Anna Maria;MESSINA, Antonella;TAROLA, Anna Maria
2001

Abstract

The separation of some anthrone-C-glucosyl constituents of aloe was carried out by isocratic capillary electrochromatography using a 50 I~m I. D. column packed with 3 I~m ODS silica gel. In comparison to HPLC mode, CEC resolution of aloins was obtained in a very short time with a high selectivi b, factor. The influence of mobile phase parameters (pH and ionic strength of the buffer, acetonitrile amount in the eluent etc.) on the retention of aloins was examined in order to improve the separation conditions and to study the retention mechanism. Efficiency was found to increase with decreasing the EOF up to a reduced plate height of about 2 at a reduced linear velocif/of 0.3 mm s 1. This effect was ascribed to the fact that, under the buffer conditions used (pH 8), aloins are partially anionically charged, with a consequent low mass transfer bel',,veen the mobile and stationary phases. However, resolution remains almost the same in a wide pH range permitting baseline separations of aloins in short analysis times. Since aloins are known to undergo fast degradation reaction dependent on pH, a kinetic study was also carried out. The method was extended to the analysis of aloins in food and cosmetic products.
2001
01 Pubblicazione su rivista::01a Articolo in rivista
Reversed-phase capillary electrochromatography of aloins and related constituents of aloe / Girelli, Anna Maria; Messina, Antonella; Ferrantelli, P.; Sinibaldi, M.; Tarola, Anna Maria. - In: CHROMATOGRAPHIA. - ISSN 0009-5893. - ELETTRONICO. - 53:(2001), pp. 284-289. [10.1007/BF02490343]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/255395
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