In the present paper we describe the preparation and characterization of reconstituted bilayer lipid membranes (BLM) supported on a polycarbonate membrane. BLMs self-assemble on the holes of the membrane, which was previously covered with An and an octadecanethiol (ODT) self-assembled monolayer (SAM). The obtained mixed hybrid bilayer lipid membranes (MHBLM) array allows recording of relatively intense signals. The steps leading to formation of the system have been investigated, its stability determined and reproducibility evaluated. The insertion of an ion channel (Gramicidin D) and a K+ carrier (Valinomycine) was also evaluated.
Membrane-supported bilayer lipid membranes array: preparation, stability and ion-channel insertion / Favero, Gabriele; D'Annibale, Andrea; Campanella, Luigi; Santucci, R; Ferri, Tommaso. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 460:(2002), pp. 23-34. [10.1016/S0003-2670(02)00139-3]
Membrane-supported bilayer lipid membranes array: preparation, stability and ion-channel insertion
FAVERO, Gabriele;D'ANNIBALE, Andrea;CAMPANELLA, Luigi;FERRI, Tommaso
2002
Abstract
In the present paper we describe the preparation and characterization of reconstituted bilayer lipid membranes (BLM) supported on a polycarbonate membrane. BLMs self-assemble on the holes of the membrane, which was previously covered with An and an octadecanethiol (ODT) self-assembled monolayer (SAM). The obtained mixed hybrid bilayer lipid membranes (MHBLM) array allows recording of relatively intense signals. The steps leading to formation of the system have been investigated, its stability determined and reproducibility evaluated. The insertion of an ion channel (Gramicidin D) and a K+ carrier (Valinomycine) was also evaluated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
