In this work data are reported on the possibility for detection of environmental contaminants endowed with toxic effects by specifically designed electrochemical biosensors. A new bienzymatic inhibition biosensor based on the combined catalytic activity of the enzymes alkaline phosphatase and glucose oxidase is proposed and discussed for the direct determination of 2,4-dichlorophenoxyacetic acid (2,4-D), one of the most powerful and diffused defoliants, which also is endowed with estrogenic properties. A discussion on the different approaches followed to manage the experimental data obtained by enzymatic inhibition biosensors is also presented.
Inhibition Based Biosensors for the Detection of Environmental Contaminants: Direct Determination of 2,4 Dichloro Phenoxyacetic Acid / Botre', Claudio; Botre', Francesco; Mazzei, Franco; Podesta', E.. - In: ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. - ISSN 0730-7268. - 19:12(2000), pp. 2876-2881. [10.1897/1551-5028(2000)019<2876:IBBFTD>2.0.CO;2]
Inhibition Based Biosensors for the Detection of Environmental Contaminants: Direct Determination of 2,4 Dichloro Phenoxyacetic Acid
BOTRE', Claudio;BOTRE', Francesco;MAZZEI, Franco;
2000
Abstract
In this work data are reported on the possibility for detection of environmental contaminants endowed with toxic effects by specifically designed electrochemical biosensors. A new bienzymatic inhibition biosensor based on the combined catalytic activity of the enzymes alkaline phosphatase and glucose oxidase is proposed and discussed for the direct determination of 2,4-dichlorophenoxyacetic acid (2,4-D), one of the most powerful and diffused defoliants, which also is endowed with estrogenic properties. A discussion on the different approaches followed to manage the experimental data obtained by enzymatic inhibition biosensors is also presented.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
