Acute effects of aflatoxins (AF), and in particular hormonal actions, have not been examined as much as chronic toxicity. Thus, we studied the effects of specific AF on prolactin (PRL) secretion by rat pituitary cells in culture. AFB(1) and AFQ(1) (1 x 10(-4) M) reduced the stimulating effect of dimethyl sulfoxide on PRL secretion by cultured rat pituitary cells. The mechanism responsible for this action is still unknown, but it did not seem to be a non specific toxic effect, because AFB(1), at the same concentration, did not significantly alter cell viability, as indicated by the Trypan blue dye-exclusion test.
Aflatoxins inhibit prolactin secretion by rat pituitary cells in culture / H., Abdel Haq; S., Giacomelli; Palmery, Maura; M. G., Leone; Saso, Luciano; Silvestrini, Bruno. - In: DRUG AND CHEMICAL TOXICOLOGY. - ISSN 0148-0545. - STAMPA. - 23:2(2000), pp. 381-386. [10.1081/dct-100100123]
Aflatoxins inhibit prolactin secretion by rat pituitary cells in culture
PALMERY, Maura;SASO, Luciano;SILVESTRINI, Bruno
2000
Abstract
Acute effects of aflatoxins (AF), and in particular hormonal actions, have not been examined as much as chronic toxicity. Thus, we studied the effects of specific AF on prolactin (PRL) secretion by rat pituitary cells in culture. AFB(1) and AFQ(1) (1 x 10(-4) M) reduced the stimulating effect of dimethyl sulfoxide on PRL secretion by cultured rat pituitary cells. The mechanism responsible for this action is still unknown, but it did not seem to be a non specific toxic effect, because AFB(1), at the same concentration, did not significantly alter cell viability, as indicated by the Trypan blue dye-exclusion test.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
