The activities of miconazole and miconazole sulphosalicylate were evaluated by a contact test using strains of Enterococcus faecalis and Escherichia coli and Candida spp selected for their resistance. The results showed that killing of the organisms occured within a few minutes. Imidazole activity required a medium of low electrical conductivity and a pH of 5.6. Greater sensitivity could be obtained with pretreatment of the microbial suspensions with sodium dioctylsulphosuccinate. Microorganisms tested in culture media with low electrical conductivity and at pH 5.6 showed enhanced sensitivity to azoles with MIC values about 20-30 times lower than those obtained using control media. Practical implications of the use of topical drugs are discussed. (C) 2001 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.
Contact imidazole activity against resistant bacteria and fungi / Simonetti, Giovanna; S., Baffa; Simonetti, Nicola. - In: INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS. - ISSN 0924-8579. - 17:5(2001), pp. 389-393. [10.1016/s0924-8579(01)00306-5]
Contact imidazole activity against resistant bacteria and fungi
SIMONETTI, Giovanna;SIMONETTI, Nicola
2001
Abstract
The activities of miconazole and miconazole sulphosalicylate were evaluated by a contact test using strains of Enterococcus faecalis and Escherichia coli and Candida spp selected for their resistance. The results showed that killing of the organisms occured within a few minutes. Imidazole activity required a medium of low electrical conductivity and a pH of 5.6. Greater sensitivity could be obtained with pretreatment of the microbial suspensions with sodium dioctylsulphosuccinate. Microorganisms tested in culture media with low electrical conductivity and at pH 5.6 showed enhanced sensitivity to azoles with MIC values about 20-30 times lower than those obtained using control media. Practical implications of the use of topical drugs are discussed. (C) 2001 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
