Lipid peroxidation results in release of 4-hydroxy-2,3-trans-nonenal (HNE), which is known to conjugate to specific amino acids of proteins and may alter their function. The effect of HNE on the activities of Na+/K(+)ATPase, Mg2+-ATPase, Ca2+-ATPase, and calmodulin-stimulated Ca2+-ATPase has been studied both in erythrocyte ghosts and in neutrophil membrane preparations. Neutrophil Ca2+-ATPase was strongly inhibited by micromolar concentrations of HNE (IC50 = 12 muM), that means in the range of pathophysiologically relevant HNE levels. The IC50 value for neutrophil Na+/K+-ATPase was about 40 muM. HNE was considerably less effective against neutrophil Mg2+-ATPase and the erythrocyte ghost enzymes (IC50 values range from 91 to 240 muM). The data suggest that HNE may play a specific role in the regulation of neutrophil calcium homeostasis in response to oxidative stress. (C) 2003 Elsevier Inc. All rights reserved.
High sensitivity of plasma membrane ion transport ATPases from human neutrophils towards 4-hydroxy-2,3-trans-nonenal / W., Siems; Capuozzo, Elisabetta; Lucano, Andrea; Salerno, Costantino; Crifo', Carlo. - In: LIFE SCIENCES. - ISSN 0024-3205. - STAMPA. - 73:20(2003), pp. 2583-2590. [10.1016/s0024-3205(03)00661-1]
High sensitivity of plasma membrane ion transport ATPases from human neutrophils towards 4-hydroxy-2,3-trans-nonenal
CAPUOZZO, Elisabetta;LUCANO, Andrea;SALERNO, Costantino;CRIFO', Carlo
2003
Abstract
Lipid peroxidation results in release of 4-hydroxy-2,3-trans-nonenal (HNE), which is known to conjugate to specific amino acids of proteins and may alter their function. The effect of HNE on the activities of Na+/K(+)ATPase, Mg2+-ATPase, Ca2+-ATPase, and calmodulin-stimulated Ca2+-ATPase has been studied both in erythrocyte ghosts and in neutrophil membrane preparations. Neutrophil Ca2+-ATPase was strongly inhibited by micromolar concentrations of HNE (IC50 = 12 muM), that means in the range of pathophysiologically relevant HNE levels. The IC50 value for neutrophil Na+/K+-ATPase was about 40 muM. HNE was considerably less effective against neutrophil Mg2+-ATPase and the erythrocyte ghost enzymes (IC50 values range from 91 to 240 muM). The data suggest that HNE may play a specific role in the regulation of neutrophil calcium homeostasis in response to oxidative stress. (C) 2003 Elsevier Inc. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
