A large body of work indicates that chromosomes play a key role in the assembly of both acentrosomal and centrosome-containing spindles. In animal systems the absence of chromosomes either prevents spindle formation or allows the assembly of a metaphase-like spindle that fails to evolve into an ana-telophase spindle. Here we show that Drosophila secondary spermatocytes can assemble morphologically normal spindles in the absence of chromosomes. The Drosophila mutants fusolo and solofuso are severely defective in chromosome segregation and produce secondary spermatocytes that are devoid of chromosomes. The centrosomes of these anucleated cells form robust asters which give rise to bipolar spindles that undergo the same ana-telophase morphological transformations that characterize normal spindles. The cells containing chromosome-free spindles are also able to assemble regular cytokinetic structures and cleave normally. In addition, chromosome-free spindles normally accumulate the Aurora B kinase at their midzones. This suggests that the association of Aurora B with chromosomes is not a prerequisite for its accumulation at the central spindle, or for its function during cytokinesis.

Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis / Elisabetta, Bucciarelli; M. G., Giansanti; Bonaccorsi, Silvia; Gatti, Maurizio. - In: THE JOURNAL OF CELL BIOLOGY. - ISSN 0021-9525. - STAMPA. - 160:7(2003), pp. 993-999. [10.1083/jcb.200211029]

Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis

BONACCORSI, Silvia;GATTI, MAURIZIO
2003

Abstract

A large body of work indicates that chromosomes play a key role in the assembly of both acentrosomal and centrosome-containing spindles. In animal systems the absence of chromosomes either prevents spindle formation or allows the assembly of a metaphase-like spindle that fails to evolve into an ana-telophase spindle. Here we show that Drosophila secondary spermatocytes can assemble morphologically normal spindles in the absence of chromosomes. The Drosophila mutants fusolo and solofuso are severely defective in chromosome segregation and produce secondary spermatocytes that are devoid of chromosomes. The centrosomes of these anucleated cells form robust asters which give rise to bipolar spindles that undergo the same ana-telophase morphological transformations that characterize normal spindles. The cells containing chromosome-free spindles are also able to assemble regular cytokinetic structures and cleave normally. In addition, chromosome-free spindles normally accumulate the Aurora B kinase at their midzones. This suggests that the association of Aurora B with chromosomes is not a prerequisite for its accumulation at the central spindle, or for its function during cytokinesis.
2003
aura b; centrosome; chromosome passengers; chromosome segregation; microtubule
01 Pubblicazione su rivista::01a Articolo in rivista
Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis / Elisabetta, Bucciarelli; M. G., Giansanti; Bonaccorsi, Silvia; Gatti, Maurizio. - In: THE JOURNAL OF CELL BIOLOGY. - ISSN 0021-9525. - STAMPA. - 160:7(2003), pp. 993-999. [10.1083/jcb.200211029]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/254377
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 26
  • Scopus 60
  • ???jsp.display-item.citation.isi??? 56
social impact