The ARS Component B gene (EMBL ID: HSARS81S, AC: X99977) encodes a 9 kD non-glycosylated polypeptide (also known as SLURP-1, SwissProt/TrEMBL: P55000), a soluble member of the human Ly-6/uPAR superfamily. ARS Component B gene mutations have been implicated in Mal de Meleda. In this study we show by immunohistochemistry that SLURP-1 (secreted Ly-6/uPAR related protein, the protein product of the ARS Component B gene) is localized to human skin, exocervix, gums, stomach and esophagus. In the epidermis, keratinocytes underlying the stratum corneum are highly positive for SLURP-1 immunostaining and cultured keratinocytes secrete the expected 9kD protein. Circulating SLURP-1 is detected in human plasma and urine. In the mouse, expression is evident in skin, eye, whole lung, trachea, esophagus and stomach. Human ARS Component B mRNA expression is regulated by retinoic acid, epidermal growth factor and interferon-γ. The tissue localization and the association with Mal de Meleda suggest that ARS Component B and its protein product SLURP-1 are implicated in maintaining the physiological and structural integrity of the keratinocyte layers of the skin.
ARS Component B: structural characterization, tissue expression and regulation of the gene and protein (SLURP-1) associated with Mal de Meleda / R., Mastrangeli; S., Donini; C. A., Kelton; C. M., He; A., Bressan; F., Milazzo; V., Ciolli; F., Borrelli; F., Martelli; M., Biffoni; O., Serlupi Crescenzi; S., Serani; E., Micangeli; N., El Tayar; Vaccaro, Rosa; Renda, Tindaro Giuseppe; R., Lisciani; M., Rossi; R., Papoian. - In: EUROPEAN JOURNAL OF DERMATOLOGY. - ISSN 1167-1122. - 13:6(2003), pp. 560-570.
ARS Component B: structural characterization, tissue expression and regulation of the gene and protein (SLURP-1) associated with Mal de Meleda
VACCARO, Rosa;RENDA, Tindaro Giuseppe;
2003
Abstract
The ARS Component B gene (EMBL ID: HSARS81S, AC: X99977) encodes a 9 kD non-glycosylated polypeptide (also known as SLURP-1, SwissProt/TrEMBL: P55000), a soluble member of the human Ly-6/uPAR superfamily. ARS Component B gene mutations have been implicated in Mal de Meleda. In this study we show by immunohistochemistry that SLURP-1 (secreted Ly-6/uPAR related protein, the protein product of the ARS Component B gene) is localized to human skin, exocervix, gums, stomach and esophagus. In the epidermis, keratinocytes underlying the stratum corneum are highly positive for SLURP-1 immunostaining and cultured keratinocytes secrete the expected 9kD protein. Circulating SLURP-1 is detected in human plasma and urine. In the mouse, expression is evident in skin, eye, whole lung, trachea, esophagus and stomach. Human ARS Component B mRNA expression is regulated by retinoic acid, epidermal growth factor and interferon-γ. The tissue localization and the association with Mal de Meleda suggest that ARS Component B and its protein product SLURP-1 are implicated in maintaining the physiological and structural integrity of the keratinocyte layers of the skin.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
