We have previously characterized a Saccharomyces cerevisiae mutant which contains a mutation in the essential rpn11/mpr1 gene coding for the proteasomal regulatory subunit Rpn11. The mpr1-1 mutation shows the phenotypic characteristics generally associated with proteasomal mutations, such as cell cycle defects and accumulation of polyubiquitinated proteins. However, for the first time, mitochondrial defects have also been found to be a consequence of a mutation in a proteasomal gene (Mol. Biol. Cell 9 (1998) 2917–2931). Since the mutant strain is thermosensitive both on glucose and on glycerol, we searched for revertants in order to shed light on the Rpn11/Mpr1 functions. Spontaneous revertants able to grow on glucose but not on glycerol at 368C were isolated, and, only from them, revertants able to grow at 368C on glycerol were selected. Revertants of the two classes were found to be extragenic. The detailed characterization of these extragenic suppressors demonstrates that the phenotypes related to cell cycle defects can be dissociated from those concerned with mitochondrial organization.

Mitochondrial effects of the pleiotropic proteasomal mutation mpr1/rpn11: uncoupling from cell cycle defects in extragenic revertants / Rinaldi, Teresa; R., Ricordy; M., Bolotin Fukuhara; Frontali, Laura. - In: GENE. - ISSN 0378-1119. - 286:1(2002), pp. 43-51. (Intervento presentato al convegno International Meeting on Mitochondria - Evolution, Genomics, Homeostasis and Pathology tenutosi a SELVA DI FASANO, ITALY nel MAY 09-12, 2001) [10.1016/s0378-1119(01)00799-5].

Mitochondrial effects of the pleiotropic proteasomal mutation mpr1/rpn11: uncoupling from cell cycle defects in extragenic revertants

RINALDI, Teresa;FRONTALI, Laura
2002

Abstract

We have previously characterized a Saccharomyces cerevisiae mutant which contains a mutation in the essential rpn11/mpr1 gene coding for the proteasomal regulatory subunit Rpn11. The mpr1-1 mutation shows the phenotypic characteristics generally associated with proteasomal mutations, such as cell cycle defects and accumulation of polyubiquitinated proteins. However, for the first time, mitochondrial defects have also been found to be a consequence of a mutation in a proteasomal gene (Mol. Biol. Cell 9 (1998) 2917–2931). Since the mutant strain is thermosensitive both on glucose and on glycerol, we searched for revertants in order to shed light on the Rpn11/Mpr1 functions. Spontaneous revertants able to grow on glucose but not on glycerol at 368C were isolated, and, only from them, revertants able to grow at 368C on glycerol were selected. Revertants of the two classes were found to be extragenic. The detailed characterization of these extragenic suppressors demonstrates that the phenotypes related to cell cycle defects can be dissociated from those concerned with mitochondrial organization.
2002
cell cycle; mitochondrial morphology; proteasome; saccharomyces cerevisiae
01 Pubblicazione su rivista::01a Articolo in rivista
Mitochondrial effects of the pleiotropic proteasomal mutation mpr1/rpn11: uncoupling from cell cycle defects in extragenic revertants / Rinaldi, Teresa; R., Ricordy; M., Bolotin Fukuhara; Frontali, Laura. - In: GENE. - ISSN 0378-1119. - 286:1(2002), pp. 43-51. (Intervento presentato al convegno International Meeting on Mitochondria - Evolution, Genomics, Homeostasis and Pathology tenutosi a SELVA DI FASANO, ITALY nel MAY 09-12, 2001) [10.1016/s0378-1119(01)00799-5].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/252744
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