The activity and substrate specificity of latex lipases from Euphorbia species (E. characias, E. wulfenii, E. pinea, E. myrsinites and E. dendroides) were investigated. High lipolytic activity was found only in E. characias and for the first time in E. wulfenii latex. For both species the lipolytic activity on various triglycerides, and under different temperature and pH conditions, in both crude latex and in partially purified enzymes was quantified. Optimised extraction and purification methods permitted the recovery of the enzymatic fraction with high lipolytic activity. This fraction is probably constituted by a pool of different lipolytic enzymes. Finally, lipolytic activity was also measured for E. characias and E. wulfenii during vegetative and reproductive stages.
Lipolytic isoenzymes from Euphorbia latex / Palocci, Cleofe; Soro, Simonetta; Cernia, Enrico; Fiorillo, Fulvio; Belsito, CARMELA MARIA ANGELICA; Monacelli, Barbara; Giuliano Delle, Monache; G. D., Monache; Pasqua, Gabriella. - In: PLANT SCIENCE. - ISSN 0168-9452. - STAMPA. - 165:3(2003), pp. 577-582. [10.1016/s0168-9452(03)00223-1]
Lipolytic isoenzymes from Euphorbia latex
PALOCCI, Cleofe;SORO, Simonetta;CERNIA, Enrico;FIORILLO, FULVIO;BELSITO, CARMELA MARIA ANGELICA;MONACELLI, Barbara;PASQUA, Gabriella
2003
Abstract
The activity and substrate specificity of latex lipases from Euphorbia species (E. characias, E. wulfenii, E. pinea, E. myrsinites and E. dendroides) were investigated. High lipolytic activity was found only in E. characias and for the first time in E. wulfenii latex. For both species the lipolytic activity on various triglycerides, and under different temperature and pH conditions, in both crude latex and in partially purified enzymes was quantified. Optimised extraction and purification methods permitted the recovery of the enzymatic fraction with high lipolytic activity. This fraction is probably constituted by a pool of different lipolytic enzymes. Finally, lipolytic activity was also measured for E. characias and E. wulfenii during vegetative and reproductive stages.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
