In this work, genomic in-situ hybridization (GISH) was used to study the sex chromosome molecular differentiation on chromosomes of male and female individuals of the isopod crustacean Asellus aquaticus. As a composite hybridization probe, we contemporaneously used male and female whole genomic DNA differently labelled in the presence of an excess of unlabelled DNA of the female homogametic sex. The karyotype of A. aquaticus normally displays eight homomorphic chromosome pairs, but a heteromorphic sex chromosome pair is present in about a quarter of the males of a natural population previously identi¢ed by us. GISH did not reveal any sex chromosome molecular differentiation on the male and female homomorphic sex chromosome pair, and the karyotypes of these individuals were equally labelled by the male- and female-derived probe, while the heteromorphic Y chromosome showed a differentially labelled region only with the male-derived probe. This region evidently contains male-speci¢c sequences but, because no similar hybridized region is observed on the male homomorphic chromosome pair, they are probably not important for sex determination but represent a molecular differentiation acquired from the Y chromosome. I.F.: 3.21
Sex chromosome differentiation revealed by comparative genomic hybridization. / Barzotti, R.; Pelliccia, Franca; Rocchi, Angela. - In: CHROMOSOME RESEARCH. - ISSN 0967-3849. - STAMPA. - 8(2000), pp. 459-464.
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|Titolo:||Sex chromosome differentiation revealed by comparative genomic hybridization.|
|Data di pubblicazione:||2000|
|Citazione:||Sex chromosome differentiation revealed by comparative genomic hybridization. / Barzotti, R.; Pelliccia, Franca; Rocchi, Angela. - In: CHROMOSOME RESEARCH. - ISSN 0967-3849. - STAMPA. - 8(2000), pp. 459-464.|
|Appartiene alla tipologia:||01a Articolo in rivista|