Four different units containing three variants of the U1 snRNA gene have been identified in the genome of Asellus aquaticus and only one unit has been identified in the genome of Proasellus coxalis. All four identified U1 snRNA genes can be folded according to the proper secondary structure and possess the functionally useful conserved sequences. Moreover, in the 3 flanking regions, all genes present both the 3 box, a conserved sequence required for 3 processing of mature snRNA, and a polyadenylation signal which is unusual for these genes. The PCR products were used as probes in fluorescent in-situ hybridization (FISH) experiments to locate them on chromosomes of A. aquaticus and P. coxalis. I.F.: 2.99
Identification and characterization of U1 small nuclear RNA genes from two crustacean isopod species / R., Barzotti; Pelliccia, Franca; Rocchi, Angela. - In: CHROMOSOME RESEARCH. - ISSN 0967-3849. - STAMPA. - 11:4(2003), pp. 365-373. [10.1023/a:1024048108918]
Identification and characterization of U1 small nuclear RNA genes from two crustacean isopod species
PELLICCIA, Franca;ROCCHI, Angela
2003
Abstract
Four different units containing three variants of the U1 snRNA gene have been identified in the genome of Asellus aquaticus and only one unit has been identified in the genome of Proasellus coxalis. All four identified U1 snRNA genes can be folded according to the proper secondary structure and possess the functionally useful conserved sequences. Moreover, in the 3 flanking regions, all genes present both the 3 box, a conserved sequence required for 3 processing of mature snRNA, and a polyadenylation signal which is unusual for these genes. The PCR products were used as probes in fluorescent in-situ hybridization (FISH) experiments to locate them on chromosomes of A. aquaticus and P. coxalis. I.F.: 2.99I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
