We report a comprehensive study of the lamellar dioleoyl trimethylammonium propane and DNA complexes (DOTAP-DNA) as a function of the lipid/DNA weight ratio rho in order to determine their structure and thermodynamic stability using an energy dispersive X-ray diffraction technique (EDXD). These self-assemblies consist of a periodic multilayer structure with DNA chains adsorbed between cationic membranes. Furthermore, above and below the isoelectric point, the DNA packing density significantly varies as a function of rho. Our results confirm that EDXD is a powerful technique to characterize self-assembled ordered structures of oppositely charged macromolecules,
SELF-ASSEMBLY OF CATIONIC LIPOSOMES-DNA COMPLEXES: A STRUCTURAL AND THERMODYNAMIC STUDY BY EDXD / Caracciolo, Giulio; Caminiti, Ruggero; Pozzi, Daniela; M., Friello; F., Boffi; Congiu, Agostina. - In: CHEMICAL PHYSICS LETTERS. - ISSN 0009-2614. - STAMPA. - 351:(2002), pp. 222-228. [10.1016/S0009-2614(01)01391-4]
SELF-ASSEMBLY OF CATIONIC LIPOSOMES-DNA COMPLEXES: A STRUCTURAL AND THERMODYNAMIC STUDY BY EDXD
CARACCIOLO, Giulio;CAMINITI, Ruggero;POZZI, DANIELA;CONGIU, Agostina
2002
Abstract
We report a comprehensive study of the lamellar dioleoyl trimethylammonium propane and DNA complexes (DOTAP-DNA) as a function of the lipid/DNA weight ratio rho in order to determine their structure and thermodynamic stability using an energy dispersive X-ray diffraction technique (EDXD). These self-assemblies consist of a periodic multilayer structure with DNA chains adsorbed between cationic membranes. Furthermore, above and below the isoelectric point, the DNA packing density significantly varies as a function of rho. Our results confirm that EDXD is a powerful technique to characterize self-assembled ordered structures of oppositely charged macromolecules,I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
