Objective.-To investigate and quantify the presence of apoptosis in early myocardial ischemia in humans. Methods.-Histologic sections from the left and right ventricles of 16 hearts with impending myocardial infarction were stained with terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) method and with antibodies to p53, bcl-2, cpp32, FAS, FAS-L, and bar. DNA electrophoretic analysis was also performed. Results.-According to the inclusion criteria, all 16 cases showed morphologic changes consistent with ischemia and/or reperfusion. TUNEL results were positive in 14 of the 16 ischemic areas. Unexpectedly, they were also positive in "remote from ischemia" myocardium of both the left and right ventricles. DNA electrophoretic analysis confirmed the results of TUNEL. Immunohistochemistry was uniformly negative, probably because of autolysis phenomena. Conclusions.-We showed that apoptosis precedes necrosis in humans, but the detection of apoptosis cannot be used as a diagnostic tool, since it can also be triggered by nonischemic events.

IS APOPTOSIS A DIAGNOSTIC MARKER OF ACUTE MYOCARDIAL INFARCTION? AN AUTOPSY STUDY COMPARING HISTOLOGICAL, IMMUNOHISTOCHEMICAL, TUNEL, AND DNA ELECTROPHORESIS EVIDENCE / Piro, Fr; DI GIOIA, Cira Rosaria Tiziana; Gallo, Pietro; Giordano, Carla; D'Amati, Giulia. - In: ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE. - ISSN 0003-9985. - STAMPA. - 124:(2000), pp. 827-831.

IS APOPTOSIS A DIAGNOSTIC MARKER OF ACUTE MYOCARDIAL INFARCTION? AN AUTOPSY STUDY COMPARING HISTOLOGICAL, IMMUNOHISTOCHEMICAL, TUNEL, AND DNA ELECTROPHORESIS EVIDENCE

DI GIOIA, Cira Rosaria Tiziana;GALLO, Pietro;GIORDANO, Carla;D'AMATI, Giulia
2000

Abstract

Objective.-To investigate and quantify the presence of apoptosis in early myocardial ischemia in humans. Methods.-Histologic sections from the left and right ventricles of 16 hearts with impending myocardial infarction were stained with terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) method and with antibodies to p53, bcl-2, cpp32, FAS, FAS-L, and bar. DNA electrophoretic analysis was also performed. Results.-According to the inclusion criteria, all 16 cases showed morphologic changes consistent with ischemia and/or reperfusion. TUNEL results were positive in 14 of the 16 ischemic areas. Unexpectedly, they were also positive in "remote from ischemia" myocardium of both the left and right ventricles. DNA electrophoretic analysis confirmed the results of TUNEL. Immunohistochemistry was uniformly negative, probably because of autolysis phenomena. Conclusions.-We showed that apoptosis precedes necrosis in humans, but the detection of apoptosis cannot be used as a diagnostic tool, since it can also be triggered by nonischemic events.
2000
01 Pubblicazione su rivista::01a Articolo in rivista
IS APOPTOSIS A DIAGNOSTIC MARKER OF ACUTE MYOCARDIAL INFARCTION? AN AUTOPSY STUDY COMPARING HISTOLOGICAL, IMMUNOHISTOCHEMICAL, TUNEL, AND DNA ELECTROPHORESIS EVIDENCE / Piro, Fr; DI GIOIA, Cira Rosaria Tiziana; Gallo, Pietro; Giordano, Carla; D'Amati, Giulia. - In: ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE. - ISSN 0003-9985. - STAMPA. - 124:(2000), pp. 827-831.
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/251037
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 34
  • ???jsp.display-item.citation.isi??? 27
social impact