Hepatocyte growth factor/scatter factor (HGF/SF) is a growth factor with pleiotropic effects on different cell types. It acts as a mitogen and motility factor for many epithelial cells. HGF/SF and its receptor Met are present in the developing and adult mammalian brain and control neuritogenesis of sympathetic and sensory neurons. We report that the striatal progenitor ST14A cells express the Met receptor, which is activated after binding with HGF/ SF. The interaction between Met and HGF/SF triggers a signaling cascade that leads to increased levels of c-Jun, c-Fos, and Egr-1 proteins, in agreement with data reported on the signaling events evoked by HGF in other cellular types. We also studied the effects of the exposure of ST1 4A cells to HGF/SF. By time-lapse photography, we observed that a 24-hr treatment with 50 ng/ml HGF/SF induced modification in cell morphology, with a decrease in cell-cell interactions and increase of cell motility. In contrast, no effect on cell proliferation was observed. To investigate which intracellular pathway is primarily involved we used PD98059 and LY294002, two specific inhibitors of mitogen-activated protein kinase/ extracellular signal-regulated kinase (MAP-kinase/ERK-kinase) and phosphoinositide 3-OH kinase (P13-K), respectively. Cell motility in HGF/SF treated cultures was inhibited by LY294002 but not by PD98059, suggesting that P13-K plays a key role in mediating the HGF/SF-induced dissociation of ST14A cells. Previous evidence of HGF stimulation of motility in nervous system has been obtained on postmitotic neurons, which have already acquired their specificity. Data reported here of a motogenic response of ST14A cell line, which displays properties of neuronal progenitors, seem of interest because they suggest that HGF could play a role in very early steps of neurogenesis. (C) 2003 Wiley-Liss, Inc.

Hepatocyte growth factor stimulates cell motility in cultures of the striatal progenitor cells ST14A / Cacci, Emanuele; M., Salani; S., Anastasi; I., Perroteau; Poiana, Giancarlo; Biagioni, Stefano; Tocco, Gabriella. - In: JOURNAL OF NEUROSCIENCE RESEARCH. - ISSN 0360-4012. - STAMPA. - 74:5(2003), pp. 760-768. [10.1002/jnr.10799]

Hepatocyte growth factor stimulates cell motility in cultures of the striatal progenitor cells ST14A

CACCI, Emanuele;POIANA, Giancarlo;BIAGIONI, Stefano;TOCCO, Gabriella
2003

Abstract

Hepatocyte growth factor/scatter factor (HGF/SF) is a growth factor with pleiotropic effects on different cell types. It acts as a mitogen and motility factor for many epithelial cells. HGF/SF and its receptor Met are present in the developing and adult mammalian brain and control neuritogenesis of sympathetic and sensory neurons. We report that the striatal progenitor ST14A cells express the Met receptor, which is activated after binding with HGF/ SF. The interaction between Met and HGF/SF triggers a signaling cascade that leads to increased levels of c-Jun, c-Fos, and Egr-1 proteins, in agreement with data reported on the signaling events evoked by HGF in other cellular types. We also studied the effects of the exposure of ST1 4A cells to HGF/SF. By time-lapse photography, we observed that a 24-hr treatment with 50 ng/ml HGF/SF induced modification in cell morphology, with a decrease in cell-cell interactions and increase of cell motility. In contrast, no effect on cell proliferation was observed. To investigate which intracellular pathway is primarily involved we used PD98059 and LY294002, two specific inhibitors of mitogen-activated protein kinase/ extracellular signal-regulated kinase (MAP-kinase/ERK-kinase) and phosphoinositide 3-OH kinase (P13-K), respectively. Cell motility in HGF/SF treated cultures was inhibited by LY294002 but not by PD98059, suggesting that P13-K plays a key role in mediating the HGF/SF-induced dissociation of ST14A cells. Previous evidence of HGF stimulation of motility in nervous system has been obtained on postmitotic neurons, which have already acquired their specificity. Data reported here of a motogenic response of ST14A cell line, which displays properties of neuronal progenitors, seem of interest because they suggest that HGF could play a role in very early steps of neurogenesis. (C) 2003 Wiley-Liss, Inc.
2003
cell motility; hepatocyte growth factor; neuron differentiation; striatal neural progenitors
01 Pubblicazione su rivista::01a Articolo in rivista
Hepatocyte growth factor stimulates cell motility in cultures of the striatal progenitor cells ST14A / Cacci, Emanuele; M., Salani; S., Anastasi; I., Perroteau; Poiana, Giancarlo; Biagioni, Stefano; Tocco, Gabriella. - In: JOURNAL OF NEUROSCIENCE RESEARCH. - ISSN 0360-4012. - STAMPA. - 74:5(2003), pp. 760-768. [10.1002/jnr.10799]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/250454
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