Here we show that apoptotic cells overexpress vinculin and are ingested by dendritic cells, which subsequently cross-prime vinculin-specific cytotoxic T lymphocytes (CTLs). Successful cross-priming requires that the apoptotic cells provide maturation signals to dendritic cells through CD40-CD40 ligand (CD40L) interactions. If apoptotic cells are CD40L-, the help of a third T cell is needed for priming, indicating a regulatory role for apoptotic cells in determining priming or tolerance. Vinculin-specific CTL priming is also related to apoptosis in vivo, given that in HIV-seropositive individuals, the frequency of specific CTLs depends on the proportion of peripheral CD40L+ apoptotic cells.
Apoptotic cells overexpress vinculin and induce vinculin-specific cytotoxic T-cell cross-priming / A., Propato; G., Cutrona; V. A. E., Francavilla; M., Ulivi; E., Schiaffella; O., Landt; R., Dunbar; E., Cerundolo; M., Ferrarini; Barnaba, Vincenzo. - In: NATURE MEDICINE. - ISSN 1078-8956. - 7:7(2001), pp. 807-813. [10.1038/89930]
Apoptotic cells overexpress vinculin and induce vinculin-specific cytotoxic T-cell cross-priming
BARNABA, Vincenzo
2001
Abstract
Here we show that apoptotic cells overexpress vinculin and are ingested by dendritic cells, which subsequently cross-prime vinculin-specific cytotoxic T lymphocytes (CTLs). Successful cross-priming requires that the apoptotic cells provide maturation signals to dendritic cells through CD40-CD40 ligand (CD40L) interactions. If apoptotic cells are CD40L-, the help of a third T cell is needed for priming, indicating a regulatory role for apoptotic cells in determining priming or tolerance. Vinculin-specific CTL priming is also related to apoptosis in vivo, given that in HIV-seropositive individuals, the frequency of specific CTLs depends on the proportion of peripheral CD40L+ apoptotic cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.