We present the first evidence of a T lymphocyte response to N-formylated peptides in humans. N-formylated peptide sequences from self (mitochondrial) and foreign (microbial) antigens were used to isolate antigen-specific T cell clones from healthy individuals, including a set of monozygotic twins. The observed response differed from that previously described in mouse (CD4(+) phenotype and MHC class II restriction in humans vs. CD8(+) phenotype and class I restriction in mice). These lymphocytes produce substantial amounts of IFN-gamma. They were isolated in only one of the monozygotic twins, which suggests that their expansion in the healthy immune repertoire is independent of the genetic background. Our result will help in assessing the relevance of N-formylated peptide-specific T cells in protection against infections within the human immune system.
T cell response to N-formylated peptides in humans / Ristori, Giovanni; Montesperelli, C; Fiorillo, Maria Teresa; Battistini, L; Chersi, A; Sorrentino, Rosa; Borsellino, G; Perna, A; Tramonti, D; Cannoni, S; Perrone, Mp; Giubilei, Franco; Riccio, P; Salvetti, Marco; Buttinelli, Carla. - In: EUROPEAN JOURNAL OF IMMUNOLOGY. - ISSN 0014-2980. - 31(9):(2001), pp. 2762-2770. [10.1002/1521-4141(200109)31:9<2762::AID-IMMU2762>3.0.CO;2-X]
T cell response to N-formylated peptides in humans
RISTORI, GIOVANNI;FIORILLO, Maria Teresa;SORRENTINO, Rosa;GIUBILEI, Franco;SALVETTI, Marco;BUTTINELLI, Carla
2001
Abstract
We present the first evidence of a T lymphocyte response to N-formylated peptides in humans. N-formylated peptide sequences from self (mitochondrial) and foreign (microbial) antigens were used to isolate antigen-specific T cell clones from healthy individuals, including a set of monozygotic twins. The observed response differed from that previously described in mouse (CD4(+) phenotype and MHC class II restriction in humans vs. CD8(+) phenotype and class I restriction in mice). These lymphocytes produce substantial amounts of IFN-gamma. They were isolated in only one of the monozygotic twins, which suggests that their expansion in the healthy immune repertoire is independent of the genetic background. Our result will help in assessing the relevance of N-formylated peptide-specific T cells in protection against infections within the human immune system.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
